Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1233087..1233589 Replicon chromosome
Accession NZ_CP063214
Organism Escherichia coli strain ERP001

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IL987_RS06250 Protein ID WP_000767829.1
Coordinates 1233087..1233341 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IL987_RS06255 Protein ID WP_001259255.1
Coordinates 1233338..1233589 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL987_RS06230 1229580..1230938 - 1359 WP_193579333.1 putrescine/proton symporter PlaP -
IL987_RS06235 1230928..1230990 - 63 WP_010723108.1 membrane protein YoeI -
IL987_RS06240 1231205..1232134 - 930 WP_000803366.1 LysR family transcriptional regulator -
IL987_RS06245 1232180..1233004 - 825 WP_021524296.1 SDR family oxidoreductase -
IL987_RS06250 1233087..1233341 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IL987_RS06255 1233338..1233589 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IL987_RS06260 1233872..1233922 + 51 WP_001364200.1 his operon leader peptide -
IL987_RS06265 1234068..1234967 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IL987_RS06270 1234973..1236277 + 1305 WP_001546022.1 histidinol dehydrogenase -
IL987_RS06275 1236274..1237344 + 1071 WP_042093612.1 histidinol-phosphate transaminase -
IL987_RS06280 1237344..1238411 + 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T177714 WP_000767829.1 NZ_CP063214:c1233341-1233087 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T177714 NZ_CP083638:c2345548-2345246 [Escherichia fergusonii]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCACTCAACGCCACATAGAAA
GATTCCTGCTGAAATCCATACCACATTATCAAGAAAATTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTATTTAGATCCACACAACTACTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT177714 WP_001259255.1 NZ_CP063214:c1233589-1233338 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT177714 NZ_CP083638:c2345210-2344869 [Escherichia fergusonii]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAGCCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACAACTG
AAATGGCGTTTCGTCTGGCTAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACACAAGAAGAATTGGGACGGATTGAAACAGTGGCTGACTATTTGGCTCGCCGTGAAGA
GCGTGCAAAGAATGTTGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References