Detailed information of TA system TA177700

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 528173..528544
• Replicon: chromosome
• Accession: NZ_CP063214
• Organism: Escherichia coli strain ERP001

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: IL987_RS02640
• Protein ID: WP_001317028.1
• Coordinates: 528350..528544 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	528173..528351 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IL987_RS02615	524128..525501	+	1374	WP_000123794.1	ATP-dependent RNA helicase DbpA	-
IL987_RS02620	525630..526565	-	936	WP_001157378.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
IL987_RS02625	526617..527852	-	1236	WP_021542962.1	site-specific integrase	-
IL987_RS02630	527854..528069	-	216	WP_000079604.1	excisionase XisR	-
IL987_RS02635	528169..528357	-	189	WP_001302840.1	DUF1187 family protein	-
-	528173..528351	+	179	NuclAT_0	-	Antitoxin
-	528173..528351	+	179	NuclAT_0	-	Antitoxin
-	528173..528351	+	179	NuclAT_0	-	Antitoxin
-	528173..528351	+	179	NuclAT_0	-	Antitoxin
IL987_RS02640	528350..528544	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
IL987_RS02645	528601..529410	-	810	WP_000166319.1	recombination protein RecT	-
IL987_RS02650	529403..532003	-	2601	WP_000105155.1	exodeoxyribonuclease VIII	-
IL987_RS02655	532105..532380	-	276	WP_021542963.1	phage protein	-
IL987_RS02660	532455..532631	-	177	WP_000245512.1	hypothetical protein	-
IL987_RS02665	532625..532846	-	222	WP_000560230.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	526617..572797	46180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T177700 WP_001317028.1 NZ_CP063214:c528544-528350 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T177700 NZ_CP083632:46426-46731 [Klebsiella quasipneumoniae]
ATGCAGTTTAAGGTATATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT177700 NZ_CP063214:528173-528351 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCCGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin