Detailed information of TA system TA177684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 818361..818953
• Replicon: chromosome
• Accession: NZ_CP063190
• Organism: Corynebacterium ihumii strain DSM 45751

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CIHUM_RS04140
• Protein ID: WP_074432060.1
• Coordinates: 818361..818639 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CIHUM_RS04145
• Protein ID: WP_034999634.1
• Coordinates: 818648..818953 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIHUM_RS04115 (CIHUM_04100)	813406..814092	-	687	WP_187370393.1	hypothetical protein	-
CIHUM_RS04120 (CIHUM_04105)	814104..815150	-	1047	WP_034999630.1	DNA recombination protein RmuC	-
CIHUM_RS04125 (CIHUM_04110)	815182..816564	-	1383	WP_243427163.1	AI-2E family transporter	-
CIHUM_RS04130 (CIHUM_04115)	816659..817744	+	1086	WP_034999632.1	redox-regulated ATPase YchF	-
CIHUM_RS04135 (CIHUM_04120)	817745..818095	-	351	WP_034999969.1	VOC family protein	-
CIHUM_RS04140	818361..818639	+	279	WP_074432060.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CIHUM_RS04145 (CIHUM_04125)	818648..818953	+	306	WP_034999634.1	HigA family addiction module antitoxin	Antitoxin
CIHUM_RS04150 (CIHUM_04130)	819030..819473	-	444	WP_034999636.1	alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB	-
CIHUM_RS04155 (CIHUM_04135)	819559..820632	-	1074	WP_051106457.1	FUSC family protein	-
CIHUM_RS04160 (CIHUM_04140)	820775..821614	+	840	WP_034999639.1	type II CAAX endopeptidase family protein	-
CIHUM_RS04165 (CIHUM_04145)	821626..822783	+	1158	WP_034999641.1	chromate efflux transporter	-
CIHUM_RS04170 (CIHUM_04150)	822844..823944	+	1101	Protein_807	Dyp-type peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-67)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10543.99 Da
• Isoelectric Point: 10.2000

>T177684 WP_074432060.1 NZ_CP063190:818361-818639 [Corynebacterium ihumii]
IQSFGNRETELVFLREPVPKLDPRIHKSANKKLHQLDAAVSLDSLLVPPGNRLEALKGDRKGTFSTRVNDQWRITFRWKG
AGPEHVTIEDYH

Nucleotide

• Length: 279 bp

>T177684 NZ_CP083630:993213-993315 [Leclercia adecarboxylata]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11296.01 Da
• Isoelectric Point: 5.0995

>AT177684 WP_034999634.1 NZ_CP063190:818648-818953 [Corynebacterium ihumii]
MSNKLYPPIHPGEILLEDFIEGFGITQHKLAVSIGVPPRRINEIVHGKRGITADTALRLGKYFGVEPIFWMNLQNQYEIE
LAEDKALAEIEQIQPVQAASA

Nucleotide

• Length: 306 bp

>AT177684 NZ_CP083630:c993317-993177 [Leclercia adecarboxylata]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAGTCGCCTTGCACTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT