Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4000494..4001293 Replicon chromosome
Accession NZ_CP063153
Organism Escherichia coli strain M7424

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag INR48_RS19050 Protein ID WP_000347269.1
Coordinates 4000829..4001293 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag INR48_RS19045 Protein ID WP_001309780.1
Coordinates 4000494..4000829 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INR48_RS19030 3996279..3997049 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
INR48_RS19035 3997065..3998399 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
INR48_RS19040 3998774..4000345 + 1572 WP_001273738.1 galactarate dehydratase -
INR48_RS19045 4000494..4000829 + 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
INR48_RS19050 4000829..4001293 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
INR48_RS19055 4001348..4002157 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
INR48_RS19060 4002406..4003686 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
INR48_RS19065 4003709..4004182 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
INR48_RS19070 4004193..4004972 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
INR48_RS19075 4004962..4005840 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
INR48_RS19080 4005858..4006292 + 435 WP_000948818.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T177659 WP_000347269.1 NZ_CP063153:4000829-4001293 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T177659 NZ_CP083613:c384860-384609 [Enterobacter hormaechei subsp. oharae]
ATGCGTGTTATTAGTTACAGTGAAGCCCGACAAAACCTGTCGGCAACCATGGTTCAGACGACGGAAGACCGCGCGCCGGT
GCTTATTACGCGCCAGAACGGTGAATCTTGTGTTCTGATGTCGCTGGAAGAGTACAACTCGCTTGAAGAAACCGCATACC
TGATGCGCTCCCCGGCAAACGCAAAACGTCTGATGAACTCTATCGAGAGCTTGAAATCTGGTAGCGGGAAGGAAAGGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT177659 WP_001309780.1 NZ_CP063153:4000494-4000829 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT177659 NZ_CP083613:c384612-384358 [Enterobacter hormaechei subsp. oharae]
GTGAAGATCACATGGTCTGAAGAGGCATGGGAAGACTACCTCTATTGGCAGGAGACCGATAAGCGCATGGTGAGGAAAAT
CAATGAGCTTATCAAAATCTCCTGTCGCACTCCGTTTGAGGGGATCGGCAAGCCAGAGCCATTAAAACATAACCTGGCTG
GCTTCTGGTCTCGCCGAATCACCTCTGAGCATCGCATGGTCTATGCCATCACAGAAGAAGCGATCCTCATAGCTGCGTGC
CGATATCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References