Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3957520..3958247 Replicon chromosome
Accession NZ_CP063153
Organism Escherichia coli strain M7424

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag INR48_RS18830 Protein ID WP_000550189.1
Coordinates 3957933..3958247 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag INR48_RS18825 Protein ID WP_000560253.1
Coordinates 3957520..3957936 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INR48_RS18815 3952935..3955286 + 2352 WP_000695439.1 alpha-glucosidase -
INR48_RS18820 3955457..3957475 + 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
INR48_RS18825 3957520..3957936 - 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
INR48_RS18830 3957933..3958247 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
INR48_RS18835 3958532..3959668 - 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
INR48_RS18840 3959753..3960256 + 504 WP_001309773.1 M48 family metallopeptidase -
INR48_RS18845 3960333..3961016 + 684 WP_001183054.1 vancomycin high temperature exclusion protein -
INR48_RS18850 3961095..3962081 + 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T177658 WP_000550189.1 NZ_CP063153:c3958247-3957933 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T177658 NZ_CP083613:c384612-384358 [Enterobacter hormaechei subsp. oharae]
GTGAAGATCACATGGTCTGAAGAGGCATGGGAAGACTACCTCTATTGGCAGGAGACCGATAAGCGCATGGTGAGGAAAAT
CAATGAGCTTATCAAAATCTCCTGTCGCACTCCGTTTGAGGGGATCGGCAAGCCAGAGCCATTAAAACATAACCTGGCTG
GCTTCTGGTCTCGCCGAATCACCTCTGAGCATCGCATGGTCTATGCCATCACAGAAGAAGCGATCCTCATAGCTGCGTGC
CGATATCACTACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT177658 WP_000560253.1 NZ_CP063153:c3957936-3957520 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT177658 NZ_CP083613:c384860-384609 [Enterobacter hormaechei subsp. oharae]
ATGCGTGTTATTAGTTACAGTGAAGCCCGACAAAACCTGTCGGCAACCATGGTTCAGACGACGGAAGACCGCGCGCCGGT
GCTTATTACGCGCCAGAACGGTGAATCTTGTGTTCTGATGTCGCTGGAAGAGTACAACTCGCTTGAAGAAACCGCATACC
TGATGCGCTCCCCGGCAAACGCAAAACGTCTGATGAACTCTATCGAGAGCTTGAAATCTGGTAGCGGGAAGGAAAGGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References