Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2147327..2147889 Replicon chromosome
Accession NZ_CP063153
Organism Escherichia coli strain M7424

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag INR48_RS10180 Protein ID WP_000605676.1
Coordinates 2147611..2147889 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag INR48_RS10175 Protein ID WP_000781370.1
Coordinates 2147327..2147611 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INR48_RS10160 2142509..2145556 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
INR48_RS10165 2145569..2146453 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
INR48_RS10170 2146446..2147099 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
INR48_RS10175 2147327..2147611 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
INR48_RS10180 2147611..2147889 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
INR48_RS10185 2148075..2149085 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
INR48_RS10190 2149219..2150916 - 1698 WP_000433464.1 malate dehydrogenase -
INR48_RS10195 2151073..2151210 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
INR48_RS10200 2151312..2151527 - 216 WP_000495766.1 biofilm-dependent modulation protein -
INR48_RS10205 2151872..2152303 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T177646 WP_000605676.1 NZ_CP063153:c2147889-2147611 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T177646 NZ_CP083610:3715289-3715576 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT177646 WP_000781370.1 NZ_CP063153:c2147611-2147327 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT177646 NZ_CP083610:3715050-3715289 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References