Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4323250..4323752 Replicon chromosome
Accession NZ_CP063046
Organism Escherichia coli strain KC-Dl-1

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag IL980_RS20770 Protein ID WP_000767828.1
Coordinates 4323498..4323752 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IL980_RS20765 Protein ID WP_001259255.1
Coordinates 4323250..4323501 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL980_RS20740 4318428..4319495 - 1068 WP_000080129.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IL980_RS20745 4319495..4320565 - 1071 WP_000108949.1 histidinol-phosphate transaminase -
IL980_RS20750 4320562..4321866 - 1305 WP_001370829.1 histidinol dehydrogenase -
IL980_RS20755 4321872..4322771 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
IL980_RS20760 4322917..4322967 - 51 WP_001364200.1 his operon leader peptide -
IL980_RS20765 4323250..4323501 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IL980_RS20770 4323498..4323752 + 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IL980_RS20775 4323835..4324659 + 825 WP_000754740.1 SDR family oxidoreductase -
IL980_RS20780 4324705..4325634 + 930 WP_000803351.1 LysR family transcriptional regulator -
IL980_RS20785 4325849..4325911 + 63 WP_010723108.1 membrane protein YoeI -
IL980_RS20790 4325901..4327259 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IL980_RS20795 4327438..4328496 + 1059 WP_000492324.1 transport protein YeeE -
IL980_RS20800 4328510..4328737 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T177550 WP_000767828.1 NZ_CP063046:4323498-4323752 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T177550 NZ_CP083530:c3887807-3887313 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT177550 WP_001259255.1 NZ_CP063046:4323250-4323501 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT177550 NZ_CP083530:c3888097-3887804 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATTTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References