Detailed information of TA system TA177549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3564141..3564868
• Replicon: chromosome
• Accession: NZ_CP063046
• Organism: Escherichia coli strain KC-Dl-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: IL980_RS17225
• Protein ID: WP_000547563.1
• Coordinates: 3564141..3564452 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IL980_RS17230
• Protein ID: WP_000126304.1
• Coordinates: 3564449..3564868 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IL980_RS17195	3559285..3560994	+	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-
IL980_RS17200	3561004..3561546	+	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
IL980_RS17205	3561546..3562313	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
IL980_RS17210	3562310..3562720	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
IL980_RS17215	3562713..3563180	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
IL980_RS17220	3563223..3563978	+	756	WP_001314074.1	hypothetical protein	-
IL980_RS17225	3564141..3564452	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
IL980_RS17230	3564449..3564868	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
IL980_RS17235	3564960..3565388	-	429	WP_000536066.1	DUF4259 domain-containing protein	-
IL980_RS17240	3565773..3566300	+	528	WP_001078777.1	electron transport protein HydN	-
IL980_RS17245	3566453..3568705	+	2253	WP_001535518.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T177549 WP_000547563.1 NZ_CP063046:3564141-3564452 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T177549 NZ_CP083530:c3633481-3633095 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT177549 WP_000126304.1 NZ_CP063046:3564449-3564868 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT177549 NZ_CP083530:c3633105-3632776 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22