Detailed information of TA system TA177519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 1..429
• Replicon: plasmid unnamed2
• Accession: NZ_CP063021
• Organism: Klebsiella pneumoniae strain CriePir75

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: GJJ10_RS27585
• Protein ID: WP_193699468.1
• Coordinates: 1..203 (-)
• Length: 67.666666666667 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A2J4ZS71
• Locus tag: GJJ10_RS27590
• Protein ID: WP_025987695.1
• Coordinates: 184..429 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJJ10_RS27585	1..203	-	203	WP_193699468.1	type II toxin-antitoxin system YoeB family toxin	Toxin
GJJ10_RS27590	184..429	-	246	WP_025987695.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GJJ10_RS27595	666..827	+	162	WP_162870772.1	hypothetical protein	-
GJJ10_RS27600	1627..2028	-	402	WP_193699469.1	helix-turn-helix domain-containing protein	-
GJJ10_RS27605	2430..3044	+	615	WP_019725132.1	TIR domain-containing protein	-
GJJ10_RS27610	3081..3788	-	708	WP_004198042.1	hypothetical protein	-
GJJ10_RS27615	3785..4690	-	906	WP_019725130.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..5010	5010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-66)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 67.666666666667 a.a.
• Molecular weight: 8040.16 Da
• Isoelectric Point: 10.5325

>T177519 WP_193699468.1 NZ_CP063021:c203-1 [Klebsiella pneumoniae]
MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGIGKPERLRHHKDPALYSRRIDQS

Nucleotide

• Length: 203 bp

>T177519 NZ_CP083512:2298011-2298475 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGATATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9361.49 Da
• Isoelectric Point: 4.4269

>AT177519 WP_025987695.1 NZ_CP063021:c429-184 [Klebsiella pneumoniae]
MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEYEALIETVYLFSNPANAEHINESMKQAERGEFVEVE
Y

Nucleotide

• Length: 246 bp

>AT177519 NZ_CP083512:2297676-2298011 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACCATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCCTGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZS71