Detailed information of TA system TA177351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 529227..529884
• Replicon: chromosome
• Accession: NZ_CP062948
• Organism: Bifidobacterium lemurum strain DSM 28807

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BL8807_RS02080
• Protein ID: WP_072723666.1
• Coordinates: 529227..529505 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BL8807_RS02085
• Protein ID: WP_072723664.1
• Coordinates: 529597..529884 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL8807_RS02070	525765..527423	+	1659	WP_072723495.1	multidrug efflux MFS transporter	-
BL8807_RS02075	528002..528916	+	915	WP_094725380.1	hypothetical protein	-
BL8807_RS02080	529227..529505	+	279	WP_072723666.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BL8807_RS02085	529597..529884	+	288	WP_072723664.1	HigA family addiction module antidote protein	Antitoxin
BL8807_RS02090	530440..531180	-	741	WP_072723492.1	HNH endonuclease family protein	-
BL8807_RS02095	531216..532091	-	876	WP_072723490.1	aldo/keto reductase	-
BL8807_RS02100	532307..534268	+	1962	WP_072723489.1	sugar porter family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-67)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 11058.59 Da
• Isoelectric Point: 10.2298

>T177351 WP_072723666.1 NZ_CP062948:529227-529505 [Bifidobacterium lemurum]
MRFADKRLERFWESGIIPSQYPQGVARTLMRKLQMLDAAEYPQALRTPPGNRFEELSGDLASHYSVRVNRQWRLVFRWDR
KEATDVRFVDYH

Nucleotide

• Length: 279 bp

>T177351 NZ_CP083448:c853451-853233 [Pantoea eucrina]
ATGAGCAATCAAACCCTGACCAAGACCGACTATTTGATGCGTCTGCGTCGTTGTCGTTCAATTGATACCCTTGAACGGGT
CATCGAAAAAAATAAGTATGAATTACCGGATAGCGAACTGGCAGTATTCTATTCAGCAGCAGATCATCGCCTTGCTGAAC
TGACCATGAACAAGCTTTACGATAAAGTGCCAGGCTCTGTGTGGAAATATGTTCGCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10391.07 Da
• Isoelectric Point: 6.7233

>AT177351 WP_072723664.1 NZ_CP062948:529597-529884 [Bifidobacterium lemurum]
MTDLLSTPGEILEEEFLSPLGISHYRLAKSIGVSQSAISDIIHGRRAITARMSLLLGMALGTGPHFWMNLQSTYDLKKAE
AEGIPTIEPLIRKNA

Nucleotide

• Length: 288 bp

>AT177351 NZ_CP083448:c853853-853476 [Pantoea eucrina]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATTCTTATGTGAAAACCTGTATGACGAGAGTATGGC
TACCCTGAGTGAAAATCATCGCGACTGGGTTAACGACCCTGCCTCCTCTGCTAATTTACAGCTCAACGATTTTATTGAGC
ATATCGCCTCTTTCACAATGAATTACAAAATTAAGCATACCGAAGACGAGTCGCTGATTTCTCAAATCGATGAATATCTT
GATGATACCTTTATGCTTTTTAGCAGTTACAGTATCAATGCAAAGGATCTTCAGCGCTGGCAGCGATCCGCTAAACGCTT
ATTTAATATTTTCGCCGAAGAGTGTGCTTATCTTCAGCAACCGAGCCATTCCTTTTAG