Detailed information of TA system TA177329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1359965..1360634
• Replicon: chromosome
• Accession: NZ_CP062938
• Organism: Bifidobacterium eulemuris strain DSM 100216

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: BE0216_RS06070
• Protein ID: WP_094635854.1
• Coordinates: 1359965..1360324 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: A0A261FRS8
• Locus tag: BE0216_RS06075
• Protein ID: WP_072724643.1
• Coordinates: 1360326..1360634 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE0216_RS06050	1355859..1357151	-	1293	WP_083570407.1	DDE-type integrase/transposase/recombinase	-
BE0216_RS06055	1357241..1357933	-	693	WP_094635851.1	hypothetical protein	-
BE0216_RS06060	1358377..1358622	-	246	WP_094635852.1	hypothetical protein	-
BE0216_RS06065	1358964..1359353	-	390	WP_094635853.1	chorismate mutase	-
BE0216_RS06070	1359965..1360324	+	360	WP_094635854.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BE0216_RS06075	1360326..1360634	+	309	WP_072724643.1	helix-turn-helix transcriptional regulator	Antitoxin
BE0216_RS06080	1361010..1363085	-	2076	WP_094635855.1	transcription termination factor Rho	-
BE0216_RS06085	1363463..1365073	-	1611	WP_094635856.1	NAD(P)/FAD-dependent oxidoreductase	-
BE0216_RS06090	1365243..1365557	-	315	WP_003828150.1	DUF2469 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1350279..1360634	10355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (11-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (25-91)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13899.01 Da
• Isoelectric Point: 9.4626

>T177329 WP_094635854.1 NZ_CP062938:1359965-1360324 [Bifidobacterium eulemuris]
MWNIVLDPIKDWLDTLDTESAEQVQAALEILRDQGPNLKRPLVGKIEGSRIVKSMKELRPGSVGRSEIRILFVFDPKRQA
IMLVGGDKQRRWNKWYKTAIEQAEARYLAWLEEQYSKEN

Nucleotide

• Length: 360 bp

>T177329 NZ_CP083443:c4814212-4813679 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11325.05 Da
• Isoelectric Point: 8.4754

>AT177329 WP_072724643.1 NZ_CP062938:1360326-1360634 [Bifidobacterium eulemuris]
MTYTIEDFARDHNTDPTKVEAAKARMLEEMRIYELKEARKAQALTQKQLAKQMGVSQKRVSIIESGDVDHVMVSTLKKYL
NGIGGSLSITAKLPSGETLELA

Nucleotide

• Length: 309 bp

>AT177329 NZ_CP083443:c4814489-4814223 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A261FRS8