Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3841232..3842031 Replicon chromosome
Accession NZ_CP062910
Organism Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag IMP05_RS18525 Protein ID WP_000347267.1
Coordinates 3841232..3841696 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IMP05_RS18530 Protein ID WP_001307405.1
Coordinates 3841696..3842031 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP05_RS18495 3836233..3836667 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IMP05_RS18500 3836685..3837563 - 879 WP_001298758.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IMP05_RS18505 3837553..3838332 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IMP05_RS18510 3838343..3838816 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IMP05_RS18515 3838839..3840119 - 1281 WP_195717708.1 tagatose-bisphosphate aldolase subunit KbaZ -
IMP05_RS18520 3840368..3841177 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IMP05_RS18525 3841232..3841696 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IMP05_RS18530 3841696..3842031 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IMP05_RS18535 3842180..3843751 - 1572 WP_001273741.1 galactarate dehydratase -
IMP05_RS18540 3844126..3845460 + 1335 WP_087889555.1 galactarate/glucarate/glycerate transporter GarP -
IMP05_RS18545 3845476..3846246 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T177294 WP_000347267.1 NZ_CP062910:c3841696-3841232 [Escherichia coli O112ab:H8]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T177294 NZ_CP083410:4352772-4352945 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT177294 WP_001307405.1 NZ_CP062910:c3842031-3841696 [Escherichia coli O112ab:H8]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT177294 NZ_CP083410:4352448-4352744 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References