Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3040567..3041169 Replicon chromosome
Accession NZ_CP062910
Organism Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag IMP05_RS14690 Protein ID WP_000897305.1
Coordinates 3040858..3041169 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IMP05_RS14685 Protein ID WP_000356397.1
Coordinates 3040567..3040857 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP05_RS14660 3036492..3037394 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
IMP05_RS14665 3037391..3038026 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
IMP05_RS14670 3038023..3038952 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
IMP05_RS14675 3039282..3039524 - 243 WP_001087409.1 hypothetical protein -
IMP05_RS14680 3039744..3039962 - 219 WP_001315930.1 ribbon-helix-helix domain-containing protein -
IMP05_RS14685 3040567..3040857 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
IMP05_RS14690 3040858..3041169 - 312 WP_000897305.1 hypothetical protein Toxin
IMP05_RS14695 3041398..3042306 + 909 WP_021293166.1 alpha/beta hydrolase -
IMP05_RS14700 3042370..3043311 - 942 WP_001343389.1 fatty acid biosynthesis protein FabY -
IMP05_RS14705 3043356..3043793 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
IMP05_RS14710 3043790..3044662 - 873 WP_195717693.1 virulence factor BrkB family protein -
IMP05_RS14715 3044656..3045255 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T177293 WP_000897305.1 NZ_CP062910:c3041169-3040858 [Escherichia coli O112ab:H8]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T177293 NZ_CP083410:c3699949-3699842 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT177293 WP_000356397.1 NZ_CP062910:c3040857-3040567 [Escherichia coli O112ab:H8]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT177293 NZ_CP083410:3699998-3700064 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References