Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 105464..105966 Replicon chromosome
Accession NZ_CP062910
Organism Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IMP05_RS00510 Protein ID WP_000767829.1
Coordinates 105712..105966 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IMP05_RS00505 Protein ID WP_001259255.1
Coordinates 105464..105715 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP05_RS00480 100642..101709 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IMP05_RS00485 101709..102779 - 1071 WP_001297360.1 histidinol-phosphate transaminase -
IMP05_RS00490 102776..104080 - 1305 WP_033873485.1 histidinol dehydrogenase -
IMP05_RS00495 104086..104985 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
IMP05_RS00500 105131..105181 - 51 WP_001364200.1 his operon leader peptide -
IMP05_RS00505 105464..105715 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IMP05_RS00510 105712..105966 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IMP05_RS00515 106049..106873 + 825 WP_000754758.1 SDR family oxidoreductase -
IMP05_RS00520 106919..107848 + 930 WP_000803351.1 LysR family transcriptional regulator -
IMP05_RS00525 108063..108125 + 63 WP_010723108.1 membrane protein YoeI -
IMP05_RS00530 108115..109473 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IMP05_RS00535 109652..110710 + 1059 WP_023568741.1 transport protein YeeE -
IMP05_RS00540 110724..110951 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T177277 WP_000767829.1 NZ_CP062910:105712-105966 [Escherichia coli O112ab:H8]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T177277 NZ_CP083410:c1270345-1270238 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT177277 WP_001259255.1 NZ_CP062910:105464-105715 [Escherichia coli O112ab:H8]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT177277 NZ_CP083410:1270392-1270458 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References