Detailed information of TA system TA177255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 728308..729001
• Replicon: chromosome
• Accession: NZ_CP062908
• Organism: Escherichia coli strain Res13-Lact-EB12-05

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: IMP23_RS04690
• Protein ID: WP_000415584.1
• Coordinates: 728308..728604 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: IMP23_RS04695
• Protein ID: WP_000650107.1
• Coordinates: 728606..729001 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP23_RS04655	723396..723710	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
IMP23_RS04660	723741..724322	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
IMP23_RS04665	724641..724973	+	333	WP_000917684.1	DUF2645 family protein	-
IMP23_RS04670	725019..726368	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
IMP23_RS04675	726365..727024	-	660	WP_001221493.1	two-component system response regulator QseB	-
IMP23_RS04680	727176..727568	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
IMP23_RS04685	727621..728103	+	483	WP_000183505.1	transcriptional regulator	-
IMP23_RS04690	728308..728604	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
IMP23_RS04695	728606..729001	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
IMP23_RS04700	729134..730741	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
IMP23_RS04705	730879..733137	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T177255 WP_000415584.1 NZ_CP062908:728308-728604 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T177255 NZ_CP083373:65532-65777 [Ensifer canadensis]
ATGAAGATCACCTACGATAACCACAAGCGCCAGTCCAACATAGCCAAGCACGGCTATGACTTTGACGATCTGCACGGCGA
GTTCTTCATGTCGTCTCTCGTGATCCCGGCAAAGGAAGGCCGCTTCATGGCAATCGGCGTCCTGCGCAACGGCGTTATCG
CAGTCGTGTTCGCCCACCTTGGAACCGAAGGCATTTCCATCATTTCCATGCGTGACGCCAGCCAGAAGGAAAGGAACCTA
CTATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT177255 WP_000650107.1 NZ_CP062908:728606-729001 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT177255 NZ_CP083373:65774-66100 [Ensifer canadensis]
ATGACCAACCAACCCCGCAAGGTTCGCTACCTCTCCGATAAAGAAGAGGCTGAAATCCAACGTAAGATAGCCAGTGACCC
CGACGCGCCAGAGGCAACAGATGATCAGTTAGCTCAAGCAAGGCCGTTTGCGGAAGCTTTCCCTGACCTGGCTGAAAGCA
TCAAGAGGTCTAGAGGGCGCCCGGCTATCGAAAAACCTCGGCAACAGATATCCATTCGGTTGGACCCCGACATCATCGAG
CACTACAAGCGCAACTGCGGCAAGGGCTGGCAAAGTCGATTGAACGACGACCTTAGAAAGGTCGTGGGATTGAAGAAAGC
CAGCTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65