Detailed information of TA system TA177223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 707734..708427
• Replicon: chromosome
• Accession: NZ_CP062901
• Organism: Escherichia coli O152:H23 strain Res13-Lact-ER01-35

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: IMP37_RS03445
• Protein ID: WP_000415584.1
• Coordinates: 707734..708030 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: IMP37_RS03450
• Protein ID: WP_000650107.1
• Coordinates: 708032..708427 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP37_RS03410	702822..703136	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
IMP37_RS03415	703167..703748	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
IMP37_RS03420	704067..704399	+	333	WP_000912120.1	DUF2645 family protein	-
IMP37_RS03425	704445..705794	-	1350	WP_000673397.1	two-component system sensor histidine kinase QseC	-
IMP37_RS03430	705791..706450	-	660	WP_001221502.1	two-component system response regulator QseB	-
IMP37_RS03435	706602..706994	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
IMP37_RS03440	707047..707529	+	483	WP_000183494.1	transcriptional regulator	-
IMP37_RS03445	707734..708030	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
IMP37_RS03450	708032..708427	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
IMP37_RS03455	708560..710167	+	1608	WP_032283533.1	ABC transporter substrate-binding protein	-
IMP37_RS03460	710305..712563	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T177223 WP_000415584.1 NZ_CP062901:707734-708030 [Escherichia coli O152:H23]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T177223 NZ_CP083350:2468816-2469325 [Ensifer adhaerens]
ATGGCGGGAAGCGGGCCGGTGCGCCTCTGGCGTGCCTATGTCCCGCGCTGGGCGCATGCGCCGCTCTCCGGTGAGGGCGC
CGCGCGCTTCGGCGGGCGCTGGAATCCTGTTGGAACACCCGCGATCTATGCTGCCCGCGAACTCTCGACCGCCTGGGCCG
AATATAATCAGGGTTTCGTCCAGCATCCGGCGCTGATCGCGCAGCTTGAGCTACGCTTCGCGCGGCTCGCTGATCTGACG
GTGGCGGAAACGCTGTCCGACCTTGGGCTCACCGAAGAGATCCACCGCTGCGAATGGCGCGATGCGCTTGATCGTGGCCT
TCTGCCGGCGACGCATCGCCTGCAGCGGGAGCTGAGCGATCGAGACTTCGATGGCCTGATCTATCCGTCCTTCATGTCGC
CCGGTGGTACCTGCGTCGCGCTCTGGCGCTGGAATGCAGGGGAGGGGGCAGAGTTGACGGTTATCGATCCTGATGGACGC
CTGCCGAAGACGCCAGCTTCCTGGCTCTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT177223 WP_000650107.1 NZ_CP062901:708032-708427 [Escherichia coli O152:H23]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT177223 NZ_CP083350:2468445-2468816 [Ensifer adhaerens]
ATGGCTGCTGTGAGTTTCAATGTCTCCGCCGCCGACTACGGCGAGCGCCAATCGTCCTTTCTCTCCGCCCGGCTGGTTGC
CGATCGCCTCGGCGTCACACTTGCCGAACTGGCGAGGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAGTCGAGTG
CCCGCAAGGTCGATGCGGCTTTGAGCGTCGTCGTGCGCATCCTCGCGATGGCCGGTGAAATGGCGGGAGACGACAGCCGC
GCCGTTATCTGGTTCAAGCACCAGCCGATCCCCGGCTGGGCCGGCAAGACCGCCTATGACCTCGTCGGCGAAGGCAAGGC
CGACAAGGTGCTCGCCTACCTCGAAGCCGTGCGTTCCGGTGTCTACGCCTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65