Detailed information of TA system TA177203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3662110..3662804
• Replicon: chromosome
• Accession: NZ_CP062895
• Organism: Escherichia coli strain Res13-Lact-ER02-39

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IMP38_RS17720
• Protein ID: WP_001263493.1
• Coordinates: 3662110..3662508 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMP38_RS17725
• Protein ID: WP_000554757.1
• Coordinates: 3662511..3662804 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP38_RS17690	3657206..3658450	-	1245	WP_000189541.1	esterase FrsA	-
-	3657866..3657946	-	81	NuclAT_10	-	-
-	3657866..3657946	-	81	NuclAT_10	-	-
-	3657866..3657946	-	81	NuclAT_10	-	-
-	3657866..3657946	-	81	NuclAT_10	-	-
IMP38_RS17695	3658542..3659000	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMP38_RS17700	3659261..3660718	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
IMP38_RS17705	3660775..3661296	-	522	Protein_3464	peptide chain release factor H	-
IMP38_RS17710	3661295..3661498	-	204	Protein_3465	RNA ligase RtcB family protein	-
IMP38_RS17715	3661744..3662100	-	357	WP_097491642.1	GNAT family N-acetyltransferase	-
IMP38_RS17720	3662110..3662508	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMP38_RS17725	3662511..3662804	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMP38_RS17730	3662856..3663911	-	1056	WP_001226164.1	DNA polymerase IV	-
IMP38_RS17735	3663982..3664767	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IMP38_RS17740	3664739..3666451	+	1713	Protein_3471	flagellar biosynthesis protein FlhA	-
IMP38_RS17745	3666675..3667172	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T177203 WP_001263493.1 NZ_CP062895:c3662508-3662110 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T177203 NZ_CP083274:3748296-3748472 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT177203 WP_000554757.1 NZ_CP062895:c3662804-3662511 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT177203 NZ_CP083274:3748518-3748934 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6