Detailed information of TA system TA177174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4449038..4449640
• Replicon: chromosome
• Accession: NZ_CP062889
• Organism: Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IMP41_RS21240
• Protein ID: WP_000897305.1
• Coordinates: 4449329..4449640 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IMP41_RS21235
• Protein ID: WP_000356397.1
• Coordinates: 4449038..4449328 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP41_RS21210	4444983..4445885	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IMP41_RS21215	4445882..4446517	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IMP41_RS21220	4446514..4447443	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IMP41_RS21225	4447773..4448015	-	243	WP_001087409.1	hypothetical protein	-
IMP41_RS21230	4448234..4448452	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
IMP41_RS21235	4449038..4449328	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IMP41_RS21240	4449329..4449640	-	312	WP_000897305.1	hypothetical protein	Toxin
IMP41_RS21245	4449869..4450777	+	909	WP_001162704.1	alpha/beta hydrolase	-
IMP41_RS21250	4450841..4451782	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IMP41_RS21255	4451827..4452264	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IMP41_RS21260	4452261..4453133	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IMP41_RS21265	4453127..4453726	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T177174 WP_000897305.1 NZ_CP062889:c4449640-4449329 [Escherichia coli O100:H21]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T177174 NZ_CP083267:2539140-2539403 [Streptomyces sp. A144]
ATGTCCATCACCGCCAGCGAAGCCCGTCAGAACCTCTTCCCGCTGATAGAGCAGGTCAACGAGGACCACGCCCCGGTGCA
CATCACCTCCCGCAAGGGGAACGCCGTGCTGATGTCCGAGGAGGACTTCACGGCGTGGACGGAGACGGTGCACCTGCTGC
GGTCGCCGAAGAACGCCCGCCGTCTGCTCGATTCGATCGCGGAGGCCGAGGCCGGCGAGGCACGGCACCGCGAGCTGATC
GACCCGGACGCGGAGCGGGCGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT177174 WP_000356397.1 NZ_CP062889:c4449328-4449038 [Escherichia coli O100:H21]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT177174 NZ_CP083267:2539400-2539654 [Streptomyces sp. A144]
GTGAGAGTCGCTTTCACGTCGCACGGCTGGGAGGACTACGTCCACTGGGCCGAGAGCGACCGGAAGATGACCAGACGGAT
CAACAAGCTGATCGCCGACATCGGGCGCGACCCCTTCAAGGGCATCGGCAAGCCGGAGCCGCTGAAGGGGGACCTGTCGG
GCTACTGGTCGCGGCGCATCGACGACACGCACCGTCTGGTGTACAAGCCGGGCGACGGCGAACTGGTCGTCGTCCAGGCG
CGGTACCACTACTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7