Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3578891..3579432 Replicon chromosome
Accession NZ_CP062889
Organism Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag IMP41_RS17145 Protein ID WP_000615982.1
Coordinates 3579154..3579432 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IMP41_RS17140 Protein ID WP_000729704.1
Coordinates 3578891..3579151 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP41_RS17105 3574629..3575492 - 864 WP_001169518.1 flagellar motor stator protein MotA -
IMP41_RS17110 3575505..3576221 - 717 WP_000938723.1 FliA/WhiG family RNA polymerase sigma factor -
IMP41_RS17115 3576241..3576708 - 468 WP_000725257.1 flagellar basal body-associated FliL family protein -
IMP41_RS17120 3576716..3577777 - 1062 WP_001067469.1 flagellar hook-length control protein FliK -
IMP41_RS17125 3577774..3578085 - 312 WP_000414996.1 hypothetical protein -
IMP41_RS17130 3578090..3578482 - 393 WP_032260988.1 flagellar export chaperone FliS -
IMP41_RS17135 3578460..3578705 - 246 Protein_3349 peptidoglycan endopeptidase -
IMP41_RS17140 3578891..3579151 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IMP41_RS17145 3579154..3579432 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IMP41_RS17150 3579588..3580328 + 741 WP_001225679.1 murein L,D-transpeptidase -
IMP41_RS17155 3580299..3581066 - 768 WP_000333380.1 class II glutamine amidotransferase -
IMP41_RS17160 3581272..3581850 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T177168 WP_000615982.1 NZ_CP062889:3579154-3579432 [Escherichia coli O100:H21]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T177168 NZ_CP083263:4197085-4197420 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT177168 WP_000729704.1 NZ_CP062889:3578891-3579151 [Escherichia coli O100:H21]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT177168 NZ_CP083263:4196837-4197085 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References