Detailed information of TA system TA177141

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 78532..78786
• Replicon: plasmid unnamednovel_0
• Accession: NZ_CP062888
• Organism: Escherichia coli strain Res13-Lact-PEA06-10

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: IMP66_RS24525
• Protein ID: WP_032339099.1
• Coordinates: 78532..78738 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	78725..78786 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP66_RS24485	73577..74727	-	1151	Protein_78	IS30 family transposase	-
IMP66_RS24490	74795..75097	-	303	Protein_79	transposase	-
IMP66_RS24495	75133..75330	-	198	Protein_80	3'-5' exonuclease	-
IMP66_RS24500	75370..75651	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IMP66_RS24505	75648..75917	-	270	WP_000079923.1	hypothetical protein	-
IMP66_RS24510	76830..77686	-	857	Protein_83	incFII family plasmid replication initiator RepA	-
IMP66_RS24515	77679..77753	-	75	WP_001365705.1	RepA leader peptide Tap	-
IMP66_RS24520	78000..78248	-	249	WP_000083838.1	replication regulatory protein RepA	-
IMP66_RS24525	78532..78738	-	207	WP_032339099.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	78725..78786	+	62	NuclAT_0	-	Antitoxin
-	78725..78786	+	62	NuclAT_0	-	Antitoxin
-	78725..78786	+	62	NuclAT_0	-	Antitoxin
-	78725..78786	+	62	NuclAT_0	-	Antitoxin
IMP66_RS24530	78961..79344	-	384	Protein_87	hypothetical protein	-
IMP66_RS24535	79462..79908	-	447	WP_032339098.1	thermonuclease family protein	-
IMP66_RS24540	80046..81320	-	1275	Protein_89	IS66-like element ISEc23 family transposase	-
IMP66_RS24550	82628..82972	-	345	Protein_91	IS66 family transposase	-
IMP66_RS24555	83003..83353	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141254	141254
	-	inside	IScluster/Tn	-	-	72662..86482	13820

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7808.29 Da
• Isoelectric Point: 8.8807

>T177141 WP_032339099.1 NZ_CP062888:c78738-78532 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYTLIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T177141 NZ_CP083240:139561-139968 [Ancylobacter polymorphus]
ATGTACCTCGTCGACACCAACATCATTTCCGAGGCCCGCCGCGGCGCTCTGCCGGCGCTCGCCTGGCTGCGCGGCGCGGA
CCCGCTCAGCGTGCATCTCAGCACCCTGACGCTCGGCGAGATCATGCGCGGCATCGCCTTGAAGCAGAGATCCGATCCGA
AGACCGCCGGGCACCTCGCCGAGTGGCTGCGCAGGATCCGCAACGATCATGCCGAGCGCATCCTGCCGGTGAGCGATCAG
ATCTCGGTCGAATGGGGCCGCATCGCCGCGATCCGCCCGCGCGGCGACATCGACGGGCTGATCGCCGCCACGGCGATCGT
CCACGACCTGATCCTGGTCACCCGCAATGTGAGAGATTTCGAGGACACCGGCGCATCGATGATCGATCCCTGGGATGTTG
AGGCATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT177141 NZ_CP062888:78725-78786 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin