Detailed information of TA system TA177130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3661629..3662323
• Replicon: chromosome
• Accession: NZ_CP062883
• Organism: Escherichia coli strain Res13-Lact-PEA06-10

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IMP66_RS17715
• Protein ID: WP_001263493.1
• Coordinates: 3661629..3662027 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMP66_RS17720
• Protein ID: WP_000554757.1
• Coordinates: 3662030..3662323 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP66_RS17685	3656725..3657969	-	1245	WP_000189541.1	esterase FrsA	-
-	3657385..3657465	-	81	NuclAT_10	-	-
-	3657385..3657465	-	81	NuclAT_10	-	-
-	3657385..3657465	-	81	NuclAT_10	-	-
-	3657385..3657465	-	81	NuclAT_10	-	-
IMP66_RS17690	3658061..3658519	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMP66_RS17695	3658780..3660237	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
IMP66_RS17700	3660294..3660815	-	522	Protein_3463	peptide chain release factor H	-
IMP66_RS17705	3660814..3661017	-	204	Protein_3464	RNA ligase RtcB family protein	-
IMP66_RS17710	3661263..3661619	-	357	WP_097491642.1	GNAT family N-acetyltransferase	-
IMP66_RS17715	3661629..3662027	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMP66_RS17720	3662030..3662323	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMP66_RS17725	3662375..3663430	-	1056	WP_001226164.1	DNA polymerase IV	-
IMP66_RS17730	3663501..3664286	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IMP66_RS17735	3664258..3665970	+	1713	Protein_3470	flagellar biosynthesis protein FlhA	-
IMP66_RS17740	3666194..3666691	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T177130 WP_001263493.1 NZ_CP062883:c3662027-3661629 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T177130 NZ_CP083182:13354-13674 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGGA
AGATCTTTTGGTACAGCCTGATAGAGGTGATCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCCCAAGGTA
ACAAAGGAAAAAGTGGTGGTATTCGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATATTTTTCTTATTGGCTTAT
CCAAAATCAGTGAAAGATAATTTAACAGATAAGGAAACAGCCATTCTGCGCCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT177130 WP_000554757.1 NZ_CP062883:c3662323-3662030 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT177130 NZ_CP083182:13667-13939 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
TCGAGTTACAGAACTCGAACTACCAGATATTAAAGAAGTACGTGAAAAAACAGGCTTGAGCCAAAATGAGTTTGCTGCAC
GTCTGCATATTAGTCCTCGTACCCTGCAAAATTGGGAACAAGGTCGACGCTATCCAACTGGACCAGCAGCTACATTAATT
CGAATTTTAGATGCTCATCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6