Detailed information of TA system TA177106

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 130290..130543
• Replicon: plasmid unnamednovel_1
• Accession: NZ_CP062879
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IMP69_RS24920
• Protein ID: WP_001312851.1
• Coordinates: 130394..130543 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	130290..130348 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS24890	126820..127566	+	747	WP_000205734.1	conjugal transfer pilus acetylase TraX	-
IMP69_RS24895	127621..128181	+	561	WP_000139340.1	fertility inhibition protein FinO	-
IMP69_RS24900	128313..128525	+	213	WP_195686733.1	hypothetical protein	-
IMP69_RS24905	128771..129232	+	462	WP_195686734.1	thermonuclease family protein	-
IMP69_RS24910	129278..129487	+	210	WP_001488746.1	hemolysin expression modulator Hha	-
IMP69_RS24915	129525..130115	+	591	WP_000766805.1	DUF2726 domain-containing protein	-
-	130290..130348	-	59	NuclAT_1	-	Antitoxin
-	130290..130348	-	59	NuclAT_1	-	Antitoxin
-	130290..130348	-	59	NuclAT_1	-	Antitoxin
-	130290..130348	-	59	NuclAT_1	-	Antitoxin
IMP69_RS24920	130394..130543	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
IMP69_RS24925	130827..131075	+	249	WP_000083832.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pic	1..131388	131388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T177106 WP_001312851.1 NZ_CP062879:130394-130543 [Escherichia coli O157:H16]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T177106 NZ_CP083116:c4834669-4834319 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT177106 NZ_CP062879:c130348-130290 [Escherichia coli O157:H16]
AGGAACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin