Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 52697..53194 Replicon plasmid unnamednovel_1
Accession NZ_CP062879
Organism Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag IMP69_RS24500 Protein ID WP_001231511.1
Coordinates 53042..53194 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IMP69_RS24495 Protein ID WP_000412343.1
Coordinates 52697..52993 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP69_RS24470 48429..49436 + 1008 WP_195686740.1 IS110 family transposase -
IMP69_RS24475 49715..49867 + 153 Protein_45 ISNCY family transposase -
IMP69_RS24480 49991..51583 - 1593 WP_000080220.1 IS66 family transposase -
IMP69_RS24485 51614..51964 - 351 WP_000624718.1 IS66 family insertion sequence element accessory protein TnpB -
IMP69_RS24490 51961..52386 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
IMP69_RS24495 52697..52993 - 297 WP_000412343.1 helix-turn-helix domain-containing protein Antitoxin
IMP69_RS24500 53042..53194 - 153 WP_001231511.1 hypothetical protein Toxin
IMP69_RS24505 53279..54447 + 1169 WP_114311086.1 IS3 family transposase -
IMP69_RS24510 54797..55963 + 1167 WP_000523852.1 plasmid-partitioning protein SopA -
IMP69_RS24515 55963..56934 + 972 WP_000817026.1 ParB/RepB/Spo0J family plasmid partition protein -
IMP69_RS24520 57447..57698 + 252 WP_001132891.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
IMP69_RS24525 57695..57982 + 288 WP_000222773.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pic 1..131388 131388
- inside IScluster/Tn - - 47078..53581 6503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-83)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5824.04 Da        Isoelectric Point: 10.8123

>T177099 WP_001231511.1 NZ_CP062879:c53194-53042 [Escherichia coli O157:H16]
MRKRGGVRVIYYVRLASGRLYLLLIYPKNAKDDLSEKEKAVMKVLAQQLK

Download         Length: 153 bp

>T177099 NZ_CP083085:c35670-35320 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11076.97 Da        Isoelectric Point: 7.2804

>AT177099 WP_000412343.1 NZ_CP062879:c52993-52697 [Escherichia coli O157:H16]
MEKKLFDALISSMEEMVAIENGQIQPAEENVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLIRSWEQKRRIPSGIALK
MLCLIEREPALINTLRSI

Download         Length: 297 bp

>AT177099 NZ_CP083085:c35962-35675 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References