Detailed information of TA system TA177092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4117201..4117928
• Replicon: chromosome
• Accession: NZ_CP062876
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: IMP69_RS20110
• Protein ID: WP_000547564.1
• Coordinates: 4117201..4117512 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IMP69_RS20115
• Protein ID: WP_000126294.1
• Coordinates: 4117509..4117928 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS20080	4112343..4114052	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
IMP69_RS20085	4114062..4114604	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
IMP69_RS20090	4114604..4115371	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
IMP69_RS20095	4115368..4115778	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
IMP69_RS20100	4115771..4116241	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
IMP69_RS20105	4116266..4117027	+	762	WP_001026446.1	hypothetical protein	-
IMP69_RS20110	4117201..4117512	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
IMP69_RS20115	4117509..4117928	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
IMP69_RS20120	4118042..4119466	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
IMP69_RS20125	4119475..4120932	-	1458	WP_001107860.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
IMP69_RS20130	4121192..4122202	+	1011	WP_001488303.1	DNA-binding transcriptional regulator AscG	-
IMP69_RS20135	4122351..4122878	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T177092 WP_000547564.1 NZ_CP062876:4117201-4117512 [Escherichia coli O157:H16]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T177092 NZ_CP083075:4222633-4222851 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT177092 WP_000126294.1 NZ_CP062876:4117509-4117928 [Escherichia coli O157:H16]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT177092 NZ_CP083075:4222232-4222606 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5