Detailed information of TA system TA177088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3639581..3640380
• Replicon: chromosome
• Accession: NZ_CP062876
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: IMP69_RS17840
• Protein ID: WP_000347273.1
• Coordinates: 3639581..3640045 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: IMP69_RS17845
• Protein ID: WP_001307405.1
• Coordinates: 3640045..3640380 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS17810	3634582..3635016	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
IMP69_RS17815	3635034..3635912	-	879	WP_001300474.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
IMP69_RS17820	3635902..3636681	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
IMP69_RS17825	3636692..3637165	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IMP69_RS17830	3637188..3638468	-	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IMP69_RS17835	3638717..3639526	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
IMP69_RS17840	3639581..3640045	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IMP69_RS17845	3640045..3640380	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IMP69_RS17850	3640529..3642100	-	1572	WP_001273753.1	galactarate dehydratase	-
IMP69_RS17855	3642475..3643809	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
IMP69_RS17860	3643825..3644595	+	771	WP_001058211.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3625897..3651150	25253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T177088 WP_000347273.1 NZ_CP062876:c3640045-3639581 [Escherichia coli O157:H16]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T177088 NZ_CP083075:1568937-1569353 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT177088 WP_001307405.1 NZ_CP062876:c3640380-3640045 [Escherichia coli O157:H16]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT177088 NZ_CP083075:1568710-1568940 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7