Detailed information of TA system TA177068

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93143..93569
• Replicon: plasmid unnamed539
• Accession: NZ_CP062874
• Organism: Escherichia coli strain Res13-Lact-PEA14-20

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IMP76_RS25490
• Protein ID: WP_001312861.1
• Coordinates: 93143..93301 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	93345..93569 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP76_RS25460	88572..89269	-	698	Protein_89	IS1 family transposase	-
IMP76_RS25465	89390..89773	-	384	WP_001151524.1	relaxosome protein TraM	-
IMP76_RS25470	90094..90696	+	603	WP_000243705.1	transglycosylase SLT domain-containing protein	-
IMP76_RS25475	90993..91814	-	822	WP_001241365.1	DUF945 domain-containing protein	-
IMP76_RS25480	91934..92221	-	288	WP_195715508.1	hypothetical protein	-
IMP76_RS25485	92246..92452	-	207	WP_033804333.1	hypothetical protein	-
IMP76_RS25490	93143..93301	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	93345..93569	-	225	NuclAT_0	-	Antitoxin
-	93345..93569	-	225	NuclAT_0	-	Antitoxin
-	93345..93569	-	225	NuclAT_0	-	Antitoxin
-	93345..93569	-	225	NuclAT_0	-	Antitoxin
IMP76_RS25495	93381..93569	+	189	WP_001299721.1	hypothetical protein	-
IMP76_RS25500	93581..94300	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
IMP76_RS25505	94297..94731	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
IMP76_RS25510	94786..96744	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
IMP76_RS25515	96803..97036	-	234	WP_000006004.1	DUF905 domain-containing protein	-
IMP76_RS25520	97092..97619	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
IMP76_RS25525	98092..98364	-	273	WP_072133095.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / exeG / exeE / stcE	1..123046	123046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T177068 WP_001312861.1 NZ_CP062874:c93301-93143 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T177068 NZ_CP083067:c56422-56138 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTTGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT177068 NZ_CP062874:c93569-93345 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin