Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3939955..3940496 Replicon chromosome
Accession NZ_CP062871
Organism Escherichia coli strain Res13-Lact-PEA14-20

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag IMP76_RS19080 Protein ID WP_000615976.1
Coordinates 3939955..3940233 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IMP76_RS19085 Protein ID WP_000729704.1
Coordinates 3940236..3940496 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP76_RS19065 3937537..3938115 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
IMP76_RS19070 3938321..3939088 + 768 WP_000333380.1 class II glutamine amidotransferase -
IMP76_RS19075 3939059..3939799 - 741 WP_195715393.1 murein L,D-transpeptidase -
IMP76_RS19080 3939955..3940233 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IMP76_RS19085 3940236..3940496 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IMP76_RS19090 3940682..3941455 + 774 WP_000543897.1 C40 family peptidase -
IMP76_RS19095 3941512..3941868 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
IMP76_RS19100 3941861..3942139 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
IMP76_RS19105 3942244..3943956 - 1713 Protein_3717 flagellar biosynthesis protein FlhA -
IMP76_RS19110 3943928..3944713 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T177059 WP_000615976.1 NZ_CP062871:c3940233-3939955 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T177059 NZ_CP083066:1882175-1882277 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT177059 WP_000729704.1 NZ_CP062871:c3940496-3940236 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT177059 NZ_CP083066:c1882284-1882139 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References