Detailed information of TA system TA177055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3041764..3042366
• Replicon: chromosome
• Accession: NZ_CP062871
• Organism: Escherichia coli strain Res13-Lact-PEA14-20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IMP76_RS14965
• Protein ID: WP_000897305.1
• Coordinates: 3041764..3042075 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IMP76_RS14970
• Protein ID: WP_000356397.1
• Coordinates: 3042076..3042366 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP76_RS14940	3037678..3038277	+	600	WP_001295269.1	glucose-1-phosphatase	-
IMP76_RS14945	3038271..3039143	+	873	WP_000920762.1	virulence factor BrkB family protein	-
IMP76_RS14950	3039140..3039577	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IMP76_RS14955	3039622..3040563	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
IMP76_RS14960	3040627..3041535	-	909	WP_001162704.1	alpha/beta hydrolase	-
IMP76_RS14965	3041764..3042075	+	312	WP_000897305.1	hypothetical protein	Toxin
IMP76_RS14970	3042076..3042366	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IMP76_RS14975	3042971..3043189	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
IMP76_RS14980	3043408..3043650	+	243	WP_001086388.1	hypothetical protein	-
IMP76_RS14985	3043879..3044859	-	981	WP_000399679.1	IS110-like element IS621 family transposase	-
IMP76_RS14990	3045258..3046187	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IMP76_RS14995	3046184..3046819	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3043879..3044859	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T177055 WP_000897305.1 NZ_CP062871:3041764-3042075 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T177055 NZ_CP083063:c5265007-5264624 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT177055 WP_000356397.1 NZ_CP062871:3042076-3042366 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT177055 NZ_CP083063:c5265249-5265007 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7