Detailed information of TA system TA177033

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 112930..113351
• Replicon: plasmid unnamed973
• Accession: NZ_CP062870
• Organism: Escherichia coli strain Res13-Lact-PEB01-20

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: IMP84_RS23550
• Protein ID: WP_001302184.1
• Coordinates: 112930..113088 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	113153..113351 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP84_RS23510	107995..108210	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
IMP84_RS23515	108346..108993	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
IMP84_RS23520	109185..109568	-	384	WP_001063020.1	relaxosome protein TraM	-
IMP84_RS23525	109920..110510	+	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
IMP84_RS23530	110805..111626	-	822	WP_001234475.1	DUF945 domain-containing protein	-
IMP84_RS23535	111745..112032	-	288	WP_000107546.1	hypothetical protein	-
IMP84_RS23540	112057..112263	-	207	WP_000547965.1	hypothetical protein	-
IMP84_RS23545	112333..112692	+	360	Protein_110	hypothetical protein	-
IMP84_RS23550	112930..113088	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	113153..113351	-	199	NuclAT_0	-	Antitoxin
-	113153..113351	-	199	NuclAT_0	-	Antitoxin
-	113153..113351	-	199	NuclAT_0	-	Antitoxin
-	113153..113351	-	199	NuclAT_0	-	Antitoxin
IMP84_RS23555	113363..114082	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
IMP84_RS23560	114079..114513	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
IMP84_RS23565	114568..115149	-	582	Protein_114	hypothetical protein	-
IMP84_RS23570	115259..116467	+	1209	WP_001352368.1	IS4-like element ISVsa5 family transposase	-
IMP84_RS23575	116482..117864	-	1383	Protein_116	ParB/RepB/Spo0J family partition protein	-
IMP84_RS23580	117930..118163	-	234	WP_000005990.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..162467	162467
	-	flank	IS/Tn	-	-	115259..116467	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T177033 WP_001302184.1 NZ_CP062870:c113088-112930 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T177033 NZ_CP083051:c5223852-5223469 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT177033 NZ_CP062870:c113351-113153 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin