Detailed information of TA system TA177023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4483016..4483618
• Replicon: chromosome
• Accession: NZ_CP062868
• Organism: Escherichia coli strain Res13-Lact-PEB01-20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IMP84_RS21390
• Protein ID: WP_000897305.1
• Coordinates: 4483307..4483618 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IMP84_RS21385
• Protein ID: WP_000356397.1
• Coordinates: 4483016..4483306 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP84_RS21360	4478961..4479863	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IMP84_RS21365	4479860..4480495	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IMP84_RS21370	4480492..4481421	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IMP84_RS21375	4481751..4481993	-	243	WP_001087409.1	hypothetical protein	-
IMP84_RS21380	4482212..4482430	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
IMP84_RS21385	4483016..4483306	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IMP84_RS21390	4483307..4483618	-	312	WP_000897305.1	hypothetical protein	Toxin
IMP84_RS21395	4483847..4484755	+	909	WP_001162704.1	alpha/beta hydrolase	-
IMP84_RS21400	4484819..4485760	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IMP84_RS21405	4485805..4486242	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IMP84_RS21410	4486239..4487111	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IMP84_RS21415	4487105..4487704	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T177023 WP_000897305.1 NZ_CP062868:c4483618-4483307 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T177023 NZ_CP083051:731006-731491 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT177023 WP_000356397.1 NZ_CP062868:c4483306-4483016 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT177023 NZ_CP083051:730716-731009 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7