Detailed information of TA system TA177018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3646886..3647427
• Replicon: chromosome
• Accession: NZ_CP062868
• Organism: Escherichia coli strain Res13-Lact-PEB01-20

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: E9TC49
• Locus tag: IMP84_RS17495
• Protein ID: WP_000615982.1
• Coordinates: 3647149..3647427 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: S1EWQ0
• Locus tag: IMP84_RS17490
• Protein ID: WP_000729704.1
• Coordinates: 3646886..3647146 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP84_RS17455	3642624..3643487	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
IMP84_RS17460	3643500..3644216	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
IMP84_RS17465	3644236..3644703	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
IMP84_RS17470	3644711..3645772	-	1062	WP_001067469.1	flagellar hook-length control protein FliK	-
IMP84_RS17475	3645769..3646080	-	312	WP_000414996.1	hypothetical protein	-
IMP84_RS17480	3646085..3646477	-	393	WP_032260988.1	flagellar export chaperone FliS	-
IMP84_RS17485	3646455..3646700	-	246	Protein_3420	peptidoglycan endopeptidase	-
IMP84_RS17490	3646886..3647146	+	261	WP_000729704.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
IMP84_RS17495	3647149..3647427	+	279	WP_000615982.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
IMP84_RS17500	3647583..3648323	+	741	WP_001225679.1	murein L,D-transpeptidase	-
IMP84_RS17505	3648294..3649061	-	768	WP_000333380.1	class II glutamine amidotransferase	-
IMP84_RS17510	3649267..3649845	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10816.59 Da
• Isoelectric Point: 10.0702

>T177018 WP_000615982.1 NZ_CP062868:3647149-3647427 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T177018 NZ_CP083048:c112804-112322 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9391.77 Da
• Isoelectric Point: 5.0758

>AT177018 WP_000729704.1 NZ_CP062868:3646886-3647146 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT177018 NZ_CP083048:c113073-112795 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• PDB: 4ML0
• AlphaFold DB: A0A0E0Y6Z6