Detailed information of TA system TA176997

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 163600..163857
• Replicon: chromosome
• Accession: NZ_CP062868
• Organism: Escherichia coli strain Res13-Lact-PEB01-20

Toxin (Protein)

• Gene name: hokA
• Uniprot ID: V0YDF1
• Locus tag: IMP84_RS00750
• Protein ID: WP_001135738.1
• Coordinates: 163705..163857 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokA
Locus tag	-
Coordinates	163600..163654 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP84_RS00730	158825..159820	-	996	WP_001182653.1	O-acetyltransferase WecH	-
IMP84_RS00735	159995..160294	+	300	Protein_145	hypothetical protein	-
IMP84_RS00740	160389..161300	+	912	WP_001168544.1	glycine--tRNA ligase subunit alpha	-
IMP84_RS00745	161310..163379	+	2070	WP_001291774.1	glycine--tRNA ligase subunit beta	-
-	163600..163654	-	55	-	-	Antitoxin
IMP84_RS00750	163705..163857	+	153	WP_001135738.1	type I toxin-antitoxin system toxin HokA	Toxin
IMP84_RS00755	164045..164257	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
IMP84_RS00760	164538..164828	-	291	WP_000455798.1	HTH-type transcriptional regulator	-
IMP84_RS00765	165262..165972	+	711	WP_000190517.1	DUF3053 domain-containing protein	-
IMP84_RS00770	166022..166996	-	975	WP_000805027.1	glyoxylate/hydroxypyruvate reductase GhrB	-
IMP84_RS00775	167100..167747	-	648	WP_000747635.1	OmpA family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (5-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5912.14 Da
• Isoelectric Point: 7.7173

>T176997 WP_001135738.1 NZ_CP062868:163705-163857 [Escherichia coli]
MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFLACNLKE

Nucleotide

• Length: 153 bp

>T176997 NZ_CP083043:c4053860-4053543 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Nucleotide

• Length: 55 bp

>AT176997 NZ_CP062868:c163654-163600 [Escherichia coli]
GTTCAGGCATAGGGTAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Structures

Toxin

• AlphaFold DB: A0A7U9LX93

Antitoxin