Detailed information of TA system TA176991

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110373..110606
• Replicon: plasmid unnamed972
• Accession: NZ_CP062867
• Organism: Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IMP90_RS24060
• Protein ID: WP_001312861.1
• Coordinates: 110373..110531 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	110575..110606 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP90_RS24035	105746..106435	-	690	WP_000283383.1	conjugal transfer transcriptional regulator TraJ	-
IMP90_RS24040	106622..107005	-	384	WP_001151528.1	relaxosome protein TraM	-
IMP90_RS24045	107326..107928	+	603	WP_000243710.1	transglycosylase SLT domain-containing protein	-
IMP90_RS24050	108225..109046	-	822	WP_001234423.1	DUF945 domain-containing protein	-
IMP90_RS24055	109166..109363	-	198	WP_052318758.1	hypothetical protein	-
IMP90_RS24060	110373..110531	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	110575..110606	-	32	NuclAT_1	-	Antitoxin
-	110575..110606	-	32	NuclAT_1	-	Antitoxin
-	110575..110606	-	32	NuclAT_1	-	Antitoxin
-	110575..110606	-	32	NuclAT_1	-	Antitoxin
-	112048..112245	-	198	NuclAT_0	-	-
-	112048..112245	-	198	NuclAT_0	-	-
-	112048..112245	-	198	NuclAT_0	-	-
-	112048..112245	-	198	NuclAT_0	-	-
IMP90_RS24070	112057..112245	+	189	WP_001299721.1	hypothetical protein	-
IMP90_RS24075	112257..112976	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
IMP90_RS24080	112973..113407	-	435	WP_000845932.1	conjugation system SOS inhibitor PsiB	-
IMP90_RS24085	113462..115420	-	1959	Protein_114	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iucA / iucB / iucC / iucD / iutA	1..155756	155756

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T176991 WP_001312861.1 NZ_CP062867:c110531-110373 [Escherichia coli O39:H21]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T176991 NZ_CP083036:c5085881-5084964 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT176991 NZ_CP062867:c110606-110575 [Escherichia coli O39:H21]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin