Detailed information of TA system TA176978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4720674..4721276
• Replicon: chromosome
• Accession: NZ_CP062865
• Organism: Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IMP90_RS22710
• Protein ID: WP_000897305.1
• Coordinates: 4720674..4720985 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IMP90_RS22715
• Protein ID: WP_000356397.1
• Coordinates: 4720986..4721276 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP90_RS22685	4716588..4717187	+	600	WP_001295269.1	glucose-1-phosphatase	-
IMP90_RS22690	4717181..4718053	+	873	WP_000920762.1	virulence factor BrkB family protein	-
IMP90_RS22695	4718050..4718487	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IMP90_RS22700	4718532..4719473	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IMP90_RS22705	4719537..4720445	-	909	WP_001162704.1	alpha/beta hydrolase	-
IMP90_RS22710	4720674..4720985	+	312	WP_000897305.1	hypothetical protein	Toxin
IMP90_RS22715	4720986..4721276	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IMP90_RS22720	4721862..4722080	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
IMP90_RS22725	4722299..4722541	+	243	WP_001087409.1	hypothetical protein	-
IMP90_RS22730	4722871..4723800	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IMP90_RS22735	4723797..4724432	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IMP90_RS22740	4724429..4725331	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T176978 WP_000897305.1 NZ_CP062865:4720674-4720985 [Escherichia coli O39:H21]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T176978 NZ_CP083033:53921-54403 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT176978 WP_000356397.1 NZ_CP062865:4720986-4721276 [Escherichia coli O39:H21]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT176978 NZ_CP083033:53667-53933 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7