Detailed information of TA system TA176953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 815716..816257
• Replicon: chromosome
• Accession: NZ_CP062865
• Organism: Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: E9TC49
• Locus tag: IMP90_RS03830
• Protein ID: WP_000615982.1
• Coordinates: 815716..815994 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: S1EWQ0
• Locus tag: IMP90_RS03835
• Protein ID: WP_000729704.1
• Coordinates: 815997..816257 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP90_RS03815	813298..813876	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
IMP90_RS03820	814082..814849	+	768	WP_000333380.1	class II glutamine amidotransferase	-
IMP90_RS03825	814820..815560	-	741	WP_001225679.1	murein L,D-transpeptidase	-
IMP90_RS03830	815716..815994	-	279	WP_000615982.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
IMP90_RS03835	815997..816257	-	261	WP_000729704.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
IMP90_RS03840	816443..816688	+	246	Protein_740	peptidoglycan endopeptidase	-
IMP90_RS03845	816666..817058	+	393	WP_032260988.1	flagellar export chaperone FliS	-
IMP90_RS03850	817063..817374	+	312	WP_000414996.1	hypothetical protein	-
IMP90_RS03855	817371..818432	+	1062	WP_001067469.1	flagellar hook-length control protein FliK	-
IMP90_RS03860	818440..818907	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
IMP90_RS03865	818927..819643	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
IMP90_RS03870	819656..820519	+	864	WP_001169518.1	flagellar motor stator protein MotA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10816.59 Da
• Isoelectric Point: 10.0702

>T176953 WP_000615982.1 NZ_CP062865:c815994-815716 [Escherichia coli O39:H21]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T176953 NZ_CP083021:c4753463-4752930 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTTGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9391.77 Da
• Isoelectric Point: 5.0758

>AT176953 WP_000729704.1 NZ_CP062865:c816257-815997 [Escherichia coli O39:H21]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT176953 NZ_CP083021:c4753740-4753474 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• PDB: 4ML0
• AlphaFold DB: A0A0E0Y6Z6