Detailed information of TA system TA176944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3678472..3679166
• Replicon: chromosome
• Accession: NZ_CP062863
• Organism: Escherichia coli strain Res13-Lact-PEB14-36

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IMP96_RS18950
• Protein ID: WP_001263493.1
• Coordinates: 3678472..3678870 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMP96_RS18955
• Protein ID: WP_000554757.1
• Coordinates: 3678873..3679166 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP96_RS18920	3673472..3674716	-	1245	WP_000189541.1	esterase FrsA	-
-	3674132..3674212	-	81	NuclAT_11	-	-
-	3674132..3674212	-	81	NuclAT_11	-	-
-	3674132..3674212	-	81	NuclAT_11	-	-
-	3674132..3674212	-	81	NuclAT_11	-	-
IMP96_RS18925	3674808..3675266	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMP96_RS18930	3675527..3676984	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IMP96_RS18935	3677041..3677562	-	522	Protein_3469	peptide chain release factor H	-
IMP96_RS18940	3677561..3677764	-	204	Protein_3470	RNA ligase RtcB family protein	-
IMP96_RS18945	3678010..3678462	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IMP96_RS18950	3678472..3678870	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMP96_RS18955	3678873..3679166	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMP96_RS18960	3679218..3680273	-	1056	WP_001226164.1	DNA polymerase IV	-
IMP96_RS18965	3680344..3681129	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
IMP96_RS18970	3681101..3682639	+	1539	Protein_3476	flagellar biosynthesis protein FlhA	-
IMP96_RS18975	3682863..3683360	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3628385..3684309	55924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T176944 WP_001263493.1 NZ_CP062863:c3678870-3678472 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176944 NZ_CP083016:c5335418-5335035 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176944 WP_000554757.1 NZ_CP062863:c3679166-3678873 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176944 NZ_CP083016:c5335660-5335418 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6