Detailed information of TA system TA176931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1797227..1797729
• Replicon: chromosome
• Accession: NZ_CP062863
• Organism: Escherichia coli strain Res13-Lact-PEB14-36

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8E0FJZ4
• Locus tag: IMP96_RS09710
• Protein ID: WP_000767833.1
• Coordinates: 1797475..1797729 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: IMP96_RS09705
• Protein ID: WP_001259255.1
• Coordinates: 1797227..1797478 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP96_RS09680	1792405..1793472	-	1068	WP_000080061.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
IMP96_RS09685	1793472..1794542	-	1071	WP_063114853.1	histidinol-phosphate transaminase	-
IMP96_RS09690	1794539..1795843	-	1305	WP_001442151.1	histidinol dehydrogenase	-
IMP96_RS09695	1795849..1796748	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
IMP96_RS09700	1796894..1796944	-	51	WP_001364200.1	his operon leader peptide	-
IMP96_RS09705	1797227..1797478	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
IMP96_RS09710	1797475..1797729	+	255	WP_000767833.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
IMP96_RS09715	1797812..1798636	+	825	WP_000754758.1	SDR family oxidoreductase	-
IMP96_RS09720	1798682..1799611	+	930	WP_000803351.1	LysR family transcriptional regulator	-
IMP96_RS09725	1799826..1799888	+	63	WP_010723108.1	membrane protein YoeI	-
IMP96_RS09730	1799878..1801236	+	1359	WP_024198437.1	putrescine/proton symporter PlaP	-
IMP96_RS09735	1801415..1802473	+	1059	WP_195714508.1	YeeE/YedE family protein	-
IMP96_RS09740	1802487..1802714	+	228	WP_000985273.1	sulfurtransferase TusA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10173.54 Da
• Isoelectric Point: 8.0353

>T176931 WP_000767833.1 NZ_CP062863:1797475-1797729 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T176931 NZ_CP083011:c4747634-4747101 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT176931 WP_001259255.1 NZ_CP062863:1797227-1797478 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT176931 NZ_CP083011:c4747911-4747645 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LNR0