Detailed information of TA system TA176912

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4146975..4147233
• Replicon: chromosome
• Accession: NZ_CP062855
• Organism: Escherichia coli O20:H12 strain Res13-Lact-PEB17-18

Toxin (Protein)

• Gene name: hokC
• Uniprot ID: -
• Locus tag: IMP99_RS20070
• Protein ID: WP_000935262.1
• Coordinates: 4147024..4147233 (+)
• Length: 70 a.a.

Antitoxin (RNA)

Gene name	sokC
Locus tag	-
Coordinates	4146975..4147032 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP99_RS20045	4142029..4142805	+	777	WP_123001070.1	fimbria/pilus outer membrane usher protein	-
IMP99_RS20050	4142980..4143354	+	375	WP_021521651.1	hypothetical protein	-
IMP99_RS20055	4143367..4144325	+	959	Protein_3925	fimbrial family protein	-
IMP99_RS20060	4144364..4145263	-	900	WP_001300855.1	transcriptional activator NhaR	-
IMP99_RS20065	4145329..4146495	-	1167	WP_000681360.1	Na+/H+ antiporter NhaA	-
-	4146975..4147032	-	58	-	-	Antitoxin
IMP99_RS20070	4147024..4147233	+	210	WP_000935262.1	type I toxin-antitoxin system Hok family toxin	Toxin
IMP99_RS20075	4147427..4148539	-	1113	WP_072643890.1	IS4-like element IS421 family transposase	-
IMP99_RS20080	4148686..4149816	-	1131	WP_001118464.1	molecular chaperone DnaJ	-
IMP99_RS20085	4149905..4151821	-	1917	WP_000516131.1	molecular chaperone DnaK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7742.28 Da
• Isoelectric Point: 9.3680

>T176912 WP_000935262.1 NZ_CP062855:4147024-4147233 [Escherichia coli O20:H12]
MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVFTAYESE

Nucleotide

• Length: 210 bp

>T176912 NZ_CP083007:351689-352036 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT176912 NZ_CP062855:c4147032-4146975 [Escherichia coli O20:H12]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Antitoxin