Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1860463..1860965 Replicon chromosome
Accession NZ_CP062855
Organism Escherichia coli O20:H12 strain Res13-Lact-PEB17-18

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IMP99_RS08865 Protein ID WP_000767829.1
Coordinates 1860711..1860965 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IMP99_RS08860 Protein ID WP_001259255.1
Coordinates 1860463..1860714 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP99_RS08835 1855641..1856708 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IMP99_RS08840 1856708..1857778 - 1071 WP_061350759.1 histidinol-phosphate transaminase -
IMP99_RS08845 1857775..1859079 - 1305 WP_001298841.1 histidinol dehydrogenase -
IMP99_RS08850 1859085..1859984 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
IMP99_RS08855 1860130..1860180 - 51 WP_001364200.1 his operon leader peptide -
IMP99_RS08860 1860463..1860714 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IMP99_RS08865 1860711..1860965 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IMP99_RS08870 1861048..1861872 + 825 WP_000754749.1 SDR family oxidoreductase -
IMP99_RS08875 1861918..1862847 + 930 WP_000803351.1 LysR family transcriptional regulator -
IMP99_RS08880 1863062..1863124 + 63 WP_010723108.1 membrane protein YoeI -
IMP99_RS08885 1863114..1864472 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IMP99_RS08890 1864651..1865709 + 1059 WP_061350760.1 transport protein YeeE -
IMP99_RS08895 1865723..1865950 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T176901 WP_000767829.1 NZ_CP062855:1860711-1860965 [Escherichia coli O20:H12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T176901 NZ_CP083000:1848705-1849037 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT176901 WP_001259255.1 NZ_CP062855:1860463-1860714 [Escherichia coli O20:H12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT176901 NZ_CP083000:1848447-1848704 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References