Detailed information of TA system TA176887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3643560..3644254
• Replicon: chromosome
• Accession: NZ_CP062853
• Organism: Escherichia coli O9:H10 strain Res13-Lact-PEB20-37-A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IMQ02_RS17820
• Protein ID: WP_001263493.1
• Coordinates: 3643560..3643958 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMQ02_RS17825
• Protein ID: WP_000554757.1
• Coordinates: 3643961..3644254 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ02_RS17790	3638560..3639804	-	1245	WP_000189541.1	esterase FrsA	-
-	3639220..3639300	-	81	NuclAT_12	-	-
-	3639220..3639300	-	81	NuclAT_12	-	-
-	3639220..3639300	-	81	NuclAT_12	-	-
-	3639220..3639300	-	81	NuclAT_12	-	-
IMQ02_RS17795	3639896..3640354	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMQ02_RS17800	3640615..3642072	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IMQ02_RS17805	3642129..3642650	-	522	Protein_3472	peptide chain release factor H	-
IMQ02_RS17810	3642649..3642852	-	204	Protein_3473	RNA ligase RtcB family protein	-
IMQ02_RS17815	3643098..3643550	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IMQ02_RS17820	3643560..3643958	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMQ02_RS17825	3643961..3644254	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMQ02_RS17830	3644306..3645361	-	1056	WP_195691080.1	DNA polymerase IV	-
IMQ02_RS17835	3645432..3646217	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
IMQ02_RS17840	3646189..3647901	+	1713	Protein_3479	flagellar biosynthesis protein FlhA	-
IMQ02_RS17845	3648117..3648614	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T176887 WP_001263493.1 NZ_CP062853:c3643958-3643560 [Escherichia coli O9:H10]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176887 NZ_CP082995:350442-350810 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176887 WP_000554757.1 NZ_CP062853:c3644254-3643961 [Escherichia coli O9:H10]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176887 NZ_CP082995:350224-350445 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6