Detailed information of TA system TA176864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3644332..3645026
• Replicon: chromosome
• Accession: NZ_CP062851
• Organism: Escherichia coli O9:H10 strain Res13-Lact-PEB21-38-A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IMQ03_RS17825
• Protein ID: WP_001263493.1
• Coordinates: 3644332..3644730 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMQ03_RS17830
• Protein ID: WP_000554757.1
• Coordinates: 3644733..3645026 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ03_RS17795	3639332..3640576	-	1245	WP_000189541.1	esterase FrsA	-
-	3639992..3640072	-	81	NuclAT_12	-	-
-	3639992..3640072	-	81	NuclAT_12	-	-
-	3639992..3640072	-	81	NuclAT_12	-	-
-	3639992..3640072	-	81	NuclAT_12	-	-
IMQ03_RS17800	3640668..3641126	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMQ03_RS17805	3641387..3642844	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IMQ03_RS17810	3642901..3643422	-	522	Protein_3473	peptide chain release factor H	-
IMQ03_RS17815	3643421..3643624	-	204	Protein_3474	RNA ligase RtcB family protein	-
IMQ03_RS17820	3643870..3644322	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IMQ03_RS17825	3644332..3644730	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMQ03_RS17830	3644733..3645026	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMQ03_RS17835	3645078..3646133	-	1056	WP_195691080.1	DNA polymerase IV	-
IMQ03_RS17840	3646204..3646989	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
IMQ03_RS17845	3646961..3648673	+	1713	Protein_3480	flagellar biosynthesis protein FlhA	-
IMQ03_RS17850	3648889..3649386	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T176864 WP_001263493.1 NZ_CP062851:c3644730-3644332 [Escherichia coli O9:H10]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176864 NZ_CP082985:4064284-4064502 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176864 WP_000554757.1 NZ_CP062851:c3645026-3644733 [Escherichia coli O9:H10]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176864 NZ_CP082985:4063883-4064257 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6