Detailed information of TA system TA176834

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65047..65311
• Replicon: plasmid unnamed476
• Accession: NZ_CP062842
• Organism: Escherichia coli strain Res13-Lact-PER02-33

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: IMQ20_RS23855
• Protein ID: WP_001331364.1
• Coordinates: 65159..65311 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	65047..65104 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ20_RS23840	60286..62577	-	2292	WP_001289276.1	hypothetical protein	-
IMQ20_RS23845	62570..63640	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
IMQ20_RS23850	63659..64867	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	65047..65104	-	58	NuclAT_0	-	Antitoxin
-	65047..65104	-	58	NuclAT_0	-	Antitoxin
-	65047..65104	-	58	NuclAT_0	-	Antitoxin
-	65047..65104	-	58	NuclAT_0	-	Antitoxin
IMQ20_RS23855	65159..65311	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
IMQ20_RS23860	65383..65634	-	252	WP_001291964.1	hypothetical protein	-
IMQ20_RS23865	66293..66469	-	177	WP_001054904.1	hypothetical protein	-
IMQ20_RS23870	66861..67070	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
IMQ20_RS23875	67142..67792	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
IMQ20_RS23880	67866..70034	-	2169	WP_021537350.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / sul2 / blaCTX-M-1	-	1..106949	106949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T176834 WP_001331364.1 NZ_CP062842:65159-65311 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T176834 NZ_CP082970:21099-21515 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT176834 NZ_CP062842:c65104-65047 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin