Detailed information of TA system TA176828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3660915..3661609
• Replicon: chromosome
• Accession: NZ_CP062838
• Organism: Escherichia coli strain Res13-Lact-PER02-33

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IMQ20_RS17700
• Protein ID: WP_001263493.1
• Coordinates: 3660915..3661313 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMQ20_RS17705
• Protein ID: WP_000554757.1
• Coordinates: 3661316..3661609 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ20_RS17670	3656011..3657255	-	1245	WP_000189541.1	esterase FrsA	-
-	3656671..3656751	-	81	NuclAT_10	-	-
-	3656671..3656751	-	81	NuclAT_10	-	-
-	3656671..3656751	-	81	NuclAT_10	-	-
-	3656671..3656751	-	81	NuclAT_10	-	-
IMQ20_RS17675	3657347..3657805	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMQ20_RS17680	3658066..3659523	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
IMQ20_RS17685	3659580..3660101	-	522	Protein_3460	peptide chain release factor H	-
IMQ20_RS17690	3660100..3660303	-	204	Protein_3461	RNA ligase RtcB family protein	-
IMQ20_RS17695	3660549..3660905	-	357	WP_097491642.1	GNAT family N-acetyltransferase	-
IMQ20_RS17700	3660915..3661313	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMQ20_RS17705	3661316..3661609	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMQ20_RS17710	3661661..3662716	-	1056	WP_001226164.1	DNA polymerase IV	-
IMQ20_RS17715	3662787..3663572	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IMQ20_RS17720	3663544..3665256	+	1713	Protein_3467	flagellar biosynthesis protein FlhA	-
IMQ20_RS17725	3665480..3665977	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3627311..3666926	39615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T176828 WP_001263493.1 NZ_CP062838:c3661313-3660915 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176828 NZ_CP082967:c4004380-4004063 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176828 WP_000554757.1 NZ_CP062838:c3661609-3661316 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176828 NZ_CP082967:c4004070-4003783 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6