Detailed information of TA system TA176792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3660932..3661626
• Replicon: chromosome
• Accession: NZ_CP062832
• Organism: Escherichia coli strain Res13-Lact-PER04-33

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IMQ22_RS17685
• Protein ID: WP_001263493.1
• Coordinates: 3660932..3661330 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMQ22_RS17690
• Protein ID: WP_000554757.1
• Coordinates: 3661333..3661626 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ22_RS17655	3656028..3657272	-	1245	WP_000189541.1	esterase FrsA	-
-	3656688..3656768	-	81	NuclAT_10	-	-
-	3656688..3656768	-	81	NuclAT_10	-	-
-	3656688..3656768	-	81	NuclAT_10	-	-
-	3656688..3656768	-	81	NuclAT_10	-	-
IMQ22_RS17660	3657364..3657822	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMQ22_RS17665	3658083..3659540	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
IMQ22_RS17670	3659597..3660118	-	522	Protein_3457	peptide chain release factor H	-
IMQ22_RS17675	3660117..3660320	-	204	Protein_3458	RNA ligase RtcB family protein	-
IMQ22_RS17680	3660566..3660922	-	357	WP_097491642.1	GNAT family N-acetyltransferase	-
IMQ22_RS17685	3660932..3661330	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMQ22_RS17690	3661333..3661626	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMQ22_RS17695	3661678..3662733	-	1056	WP_001226164.1	DNA polymerase IV	-
IMQ22_RS17700	3662804..3663589	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IMQ22_RS17705	3663561..3665273	+	1713	Protein_3464	flagellar biosynthesis protein FlhA	-
IMQ22_RS17710	3665497..3665994	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T176792 WP_001263493.1 NZ_CP062832:c3661330-3660932 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176792 NZ_CP082938:c274784-274368 [Klebsiella pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTGCTGAAGCGCCT
GGAGCAGGCAGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATTACCTACTCTGAAATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCACCGCGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGCGTCGATGCCATCCTGCCCTGGGATCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCGCTGCGCCTCGCAGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCACGCCATCGCCGCCGGCGCCGTACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTCCTAGGGCTGAATCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176792 WP_000554757.1 NZ_CP062832:c3661626-3661333 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176792 NZ_CP082938:c275011-274781 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATACGTCTGCCCCGGGACCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTTCGCGAAGGGGACAGTATCATCCTGCGTCCCGTCCGGCCGACCTGGGGTTCATTCGCCAGCCTTG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6