Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 661007..661734 Replicon chromosome
Accession NZ_CP062832
Organism Escherichia coli strain Res13-Lact-PER04-33

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IMQ22_RS03205 Protein ID WP_000550189.1
Coordinates 661007..661321 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IMQ22_RS03210 Protein ID WP_000560266.1
Coordinates 661318..661734 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ22_RS03185 657165..658151 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
IMQ22_RS03190 658230..658922 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
IMQ22_RS03195 658999..659502 - 504 WP_001300832.1 M48 family metallopeptidase -
IMQ22_RS03200 659587..660723 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IMQ22_RS03205 661007..661321 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IMQ22_RS03210 661318..661734 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IMQ22_RS03215 661779..663797 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IMQ22_RS03220 664223..666574 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T176775 WP_000550189.1 NZ_CP062832:661007-661321 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T176775 NZ_CP082929:4476083-4476394 [Salmonella enterica subsp. enterica serovar London]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGCACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT176775 WP_000560266.1 NZ_CP062832:661318-661734 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT176775 NZ_CP082929:4476391-4476837 [Salmonella enterica subsp. enterica serovar London]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References