Detailed information of TA system TA176756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3660108..3660802
• Replicon: chromosome
• Accession: NZ_CP062826
• Organism: Escherichia coli strain Res13-Lact-PER12-33-A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IMQ30_RS17690
• Protein ID: WP_001263493.1
• Coordinates: 3660108..3660506 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMQ30_RS17695
• Protein ID: WP_000554757.1
• Coordinates: 3660509..3660802 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ30_RS17660	3655204..3656448	-	1245	WP_000189541.1	esterase FrsA	-
-	3655864..3655944	-	81	NuclAT_10	-	-
-	3655864..3655944	-	81	NuclAT_10	-	-
-	3655864..3655944	-	81	NuclAT_10	-	-
-	3655864..3655944	-	81	NuclAT_10	-	-
IMQ30_RS17665	3656540..3656998	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMQ30_RS17670	3657259..3658716	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
IMQ30_RS17675	3658773..3659294	-	522	Protein_3458	peptide chain release factor H	-
IMQ30_RS17680	3659293..3659496	-	204	Protein_3459	RNA ligase RtcB family protein	-
IMQ30_RS17685	3659742..3660098	-	357	WP_097491642.1	GNAT family N-acetyltransferase	-
IMQ30_RS17690	3660108..3660506	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMQ30_RS17695	3660509..3660802	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMQ30_RS17700	3660854..3661909	-	1056	WP_001226164.1	DNA polymerase IV	-
IMQ30_RS17705	3661980..3662765	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IMQ30_RS17710	3662737..3664449	+	1713	Protein_3465	flagellar biosynthesis protein FlhA	-
IMQ30_RS17715	3664673..3665170	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T176756 WP_001263493.1 NZ_CP062826:c3660506-3660108 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176756 NZ_CP082926:c3962730-3962197 [Shewanella inventionis]
GTGAGTTGGGGTAAAGAGTTCGTAGAACTTAGTAAATCATATCACGACCGCAATTCATTCGACTGTGGTGAGCAAGAGCT
AAATACATTTATCAAAACTCAAGCAGCAAAGCATATGGAAGCAGGTATTAGTCGAACAATGATTCTCCCTAGTGCTCAGC
CATTGCTTAATCAGAAGTTTGCAATATGTGCATTTTACAGTGTTGCGCCTAGCTCAATCAATCGAGAAACCTTACCAGAG
CAACTAGCAAAGAAACTTCCTCGATACCCAATTCCAGTGTTTCTTTTAGCTCAGTTAGCTGTGCATAAGGAATTTCACGG
CTCTGGGCTAGGAAAAGTCAGTTTAATCCGTGCTCTGAAGTACCTTTGGGAAGTTAACCACCATATGAGGGCTTATGCTA
TTGTCGTGGACTGTCTCACTGATCCAGCACAGGCATTCTATACAAAATTTGGCTTTGAAGTATTGTGTGAACATAATGGA
CGCATTCGCATGTTTCTACCAATGAAAACGATAGAAAGACTTTTTAACCAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176756 WP_000554757.1 NZ_CP062826:c3660802-3660509 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176756 NZ_CP082926:c3962996-3962727 [Shewanella inventionis]
ATGGCAACAGCACGTTTAGATATCCGTCTTGATGAAGAGATAAAAGCGAAAGCAGAAAAGGCATCAGCATTGCTTGGTCT
GAAAAGTTTAACTGAATATGTAGTTAGGCTTATGGACGAAGATGCAACAAACGTAATTGAGAAGCACGAAGGTATCGTGG
TTCAAGATAGTGTCTTTGACGCATTTATGGCTGCTTGTAACAAAGCAAAATCGCCAAATCAAGCTTTGTTGAATGCTGCA
AAATTCACAGATGAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6