Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4198180..4198907 Replicon chromosome
Accession NZ_CP062824
Organism Escherichia coli O167:H26 strain Res13-Lact-PER13-34

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag IMQ31_RS19920 Protein ID WP_000547555.1
Coordinates 4198180..4198491 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1M2G9D6
Locus tag IMQ31_RS19925 Protein ID WP_001600394.1
Coordinates 4198488..4198907 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ31_RS19895 4194115..4195824 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
IMQ31_RS19900 4195834..4196376 + 543 WP_195714873.1 formate hydrogenlyase complex iron-sulfur subunit -
IMQ31_RS19905 4196376..4197143 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
IMQ31_RS19910 4197140..4197550 + 411 WP_085458983.1 formate hydrogenlyase assembly protein HycH -
IMQ31_RS19915 4197543..4198013 + 471 WP_085458982.1 hydrogenase maturation peptidase HycI -
IMQ31_RS19920 4198180..4198491 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
IMQ31_RS19925 4198488..4198907 + 420 WP_001600394.1 helix-turn-helix domain-containing protein Antitoxin
IMQ31_RS19930 4198986..4200410 - 1425 WP_085459082.1 6-phospho-beta-glucosidase AscB -
IMQ31_RS19935 4200419..4201876 - 1458 WP_085458981.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
IMQ31_RS19940 4202136..4203146 + 1011 WP_024187449.1 DNA-binding transcriptional regulator AscG -
IMQ31_RS19945 4203295..4203822 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T176732 WP_000547555.1 NZ_CP062824:4198180-4198491 [Escherichia coli O167:H26]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T176732 NZ_CP082922:c1232924-1232523 [Nocardioides rotundus]
GTGATCGTCCTCGACGCCTCGGCCGCGGTGGAGTGGCTGCTGGCACGCCCGGATGCCGCAGCCGTGGCGACGAGGATCAA
CGATCCCGATGTCGCGCTGCATGCGCCCTCGCTGTTGGGGGTCGAGGTCGCGGCGGCTCTGCGCGGTCTGGTCCGCGGCG
GTCACGCCTCGCCCGAGCGGGCTGTCACCGCTCTTGAGGACCTTGCCGCAGCCGGCATCGCGCTGCACGACCCGAGCCCG
CTGCTCCCGCGAGCGTGGGAGCTGCGGGACAACCTGACGCCCTATGACGCGGTCTACGTCGCCCTGGCCGAGGTGCTGGA
CGCGACCCTGGTCACCGCCGACGCCAGGATCGCCCGGGCCCCCGGGCTGCAAGCACCCGTCGACGTCGTCACGAGTCACT
GA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15389.40 Da        Isoelectric Point: 4.4596

>AT176732 WP_001600394.1 NZ_CP062824:4198488-4198907 [Escherichia coli O167:H26]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYESNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT176732 NZ_CP082922:c1233148-1232921 [Nocardioides rotundus]
ATGAGCATGATCCAGATCCGCAACGTCCCCGAGGACGTCCACCGGCGCCTCAAGGCCAGGGCGGCGTTGGAGGGGGTCTC
CCTCTCCGAGCTCGCCCTGGCCGAGCTGCGTCGCTCCCTGGACCGGCCCACACGGCAGGAGCTGCTCGACCTGGTGGCGT
CCCGGACGCCGGTGCGCACGGGGCCGTCCTCGGCGGAGCTCGTCGAGGCGAGTCGCGCCGACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2G9D6

References