Detailed information of TA system TA176727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2849938..2850540
• Replicon: chromosome
• Accession: NZ_CP062824
• Organism: Escherichia coli O167:H26 strain Res13-Lact-PER13-34

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IMQ31_RS13545
• Protein ID: WP_000897305.1
• Coordinates: 2850229..2850540 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IMQ31_RS13540
• Protein ID: WP_000356395.1
• Coordinates: 2849938..2850228 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ31_RS13505	2845561..2846463	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IMQ31_RS13510	2846460..2847095	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IMQ31_RS13515	2847092..2848021	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IMQ31_RS13520	2848203..2848445	-	243	WP_001086386.1	ribbon-helix-helix domain-containing protein	-
IMQ31_RS13525	2848664..2848882	-	219	WP_001315113.1	ribbon-helix-helix domain-containing protein	-
IMQ31_RS13530	2849301..2849579	-	279	WP_001315112.1	hypothetical protein	-
IMQ31_RS13535	2849641..2849853	-	213	WP_000197769.1	hypothetical protein	-
IMQ31_RS13540	2849938..2850228	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
IMQ31_RS13545	2850229..2850540	-	312	WP_000897305.1	hypothetical protein	Toxin
IMQ31_RS13550	2850769..2851677	+	909	WP_001306651.1	alpha/beta hydrolase	-
IMQ31_RS13555	2851846..2853660	-	1815	WP_000226339.1	hypothetical protein	-
IMQ31_RS13560	2854076..2855017	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IMQ31_RS13565	2855062..2855499	-	438	WP_195714826.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T176727 WP_000897305.1 NZ_CP062824:c2850540-2850229 [Escherichia coli O167:H26]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T176727 NZ_CP082916:c3254168-3253641 [Salmonella enterica subsp. enterica serovar Newlands]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT176727 WP_000356395.1 NZ_CP062824:c2850228-2849938 [Escherichia coli O167:H26]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT176727 NZ_CP082916:c3254455-3254165 [Salmonella enterica subsp. enterica serovar Newlands]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7