Detailed information of TA system TA176693

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 78537..78791
• Replicon: plasmid unnamednovel_0
• Accession: NZ_CP062820
• Organism: Escherichia coli strain Res13-Sevr-PER06-05-b-A

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: IMQ58_RS24515
• Protein ID: WP_032339099.1
• Coordinates: 78537..78743 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	78730..78791 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ58_RS24475	73578..74729	-	1152	WP_195706813.1	IS30 family transposase	-
IMQ58_RS24480	74797..75099	-	303	Protein_79	transposase	-
IMQ58_RS24485	75135..75332	-	198	Protein_80	3'-5' exonuclease	-
IMQ58_RS24490	75372..75653	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IMQ58_RS24495	75650..75919	-	270	WP_000079923.1	hypothetical protein	-
IMQ58_RS24500	76835..77691	-	857	Protein_83	incFII family plasmid replication initiator RepA	-
IMQ58_RS24505	77684..77758	-	75	WP_001365705.1	RepA leader peptide Tap	-
IMQ58_RS24510	78005..78253	-	249	WP_000083838.1	replication regulatory protein RepA	-
IMQ58_RS24515	78537..78743	-	207	WP_032339099.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	78730..78791	+	62	NuclAT_0	-	Antitoxin
-	78730..78791	+	62	NuclAT_0	-	Antitoxin
-	78730..78791	+	62	NuclAT_0	-	Antitoxin
-	78730..78791	+	62	NuclAT_0	-	Antitoxin
IMQ58_RS24520	78966..79349	-	384	Protein_87	hypothetical protein	-
IMQ58_RS24525	79467..79913	-	447	WP_032339098.1	thermonuclease family protein	-
IMQ58_RS24530	80051..81325	-	1275	Protein_89	IS66-like element ISEc23 family transposase	-
IMQ58_RS24540	82633..82977	-	345	Protein_91	IS66 family transposase	-
IMQ58_RS24545	83008..83358	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141259	141259
	-	inside	IScluster/Tn	-	-	72663..86487	13824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7808.29 Da
• Isoelectric Point: 8.8807

>T176693 WP_032339099.1 NZ_CP062820:c78743-78537 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYTLIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T176693 NZ_CP082909:1682683-1683105 [Endozoicomonas sp. 4G]
ATGAATGACATTATTCTTAACAAATACTCAATCATTCAGCGTTGTATTGCCCGTATTAACGAAGAATACAAAGGTCATGA
GTCTGAACTGTGCACAAATTACACTCAGCAGGATTCTATAATTCTGAATATTCAGAGAGCTTGCCAGGCTGCCCAGGATA
TGGCGAACTACGTTATCAAACTGAAAAAATTGGGAATCCCCCAGAATGCCAGAGAATCTTTCCAGCTACTAAACGATCAT
GGACTGGTATCAGCCCCACTCACAATCAGTATGATAAAAATGGTTGGGTTCAGGAATATAGCGGTCCACGAGTACCAAAA
ATTGAACATAGCTATTCTTAAATCTGTTATAGAAAAGCATGTTGATGAAATTAACTGGTTCGCAGAAGAAGTTATGCGCA
GCTTTAATAATGATGGACAATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT176693 NZ_CP062820:78730-78791 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin