Detailed information of TA system TA176682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3659583..3660277
• Replicon: chromosome
• Accession: NZ_CP062815
• Organism: Escherichia coli strain Res13-Sevr-PER06-05-b-A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IMQ58_RS17705
• Protein ID: WP_001263493.1
• Coordinates: 3659583..3659981 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMQ58_RS17710
• Protein ID: WP_000554757.1
• Coordinates: 3659984..3660277 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ58_RS17675	3654679..3655923	-	1245	WP_000189541.1	esterase FrsA	-
-	3655339..3655419	-	81	NuclAT_10	-	-
-	3655339..3655419	-	81	NuclAT_10	-	-
-	3655339..3655419	-	81	NuclAT_10	-	-
-	3655339..3655419	-	81	NuclAT_10	-	-
IMQ58_RS17680	3656015..3656473	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMQ58_RS17685	3656734..3658191	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
IMQ58_RS17690	3658248..3658769	-	522	Protein_3461	peptide chain release factor H	-
IMQ58_RS17695	3658768..3658971	-	204	Protein_3462	RNA ligase RtcB family protein	-
IMQ58_RS17700	3659217..3659573	-	357	WP_097491642.1	GNAT family N-acetyltransferase	-
IMQ58_RS17705	3659583..3659981	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMQ58_RS17710	3659984..3660277	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMQ58_RS17715	3660329..3661384	-	1056	WP_001226164.1	DNA polymerase IV	-
IMQ58_RS17720	3661455..3662240	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IMQ58_RS17725	3662212..3663924	+	1713	Protein_3468	flagellar biosynthesis protein FlhA	-
IMQ58_RS17730	3664148..3664645	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T176682 WP_001263493.1 NZ_CP062815:c3659981-3659583 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176682 NZ_CP082909:148719-148991 [Endozoicomonas sp. 4G]
ATGACTGAGGGGATTAGTATGGAAAATCAATGGCAGTTACAGGAGGCGAAAAGCAAGTTCAGTCATATTGTGGACAGGGC
TCGTCAGCATGGTCCACAATTCGTGACCCGTCATGGGCATAAAGCGGCTGTCGTCCTCTCATTTGAAGACTATCAAAGGA
TGTGTGCACCAGAGAATAGTCTGAGTGAGTTTCTTAAAGCCTCACCCTTTTCAGATTCTGATGTTGAAACGTTTGACAGA
AGTAAAGAAACCCCGAGGGATGTAGATCTGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176682 WP_000554757.1 NZ_CP062815:c3660277-3659984 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176682 NZ_CP082909:148988-149434 [Endozoicomonas sp. 4G]
GTGAAGTATTTACTGGATACCTGCGTGTTGTCAGAAGCATTCAAAAAAAATCCTGATAAGCATGTACTTGAATGGATCGA
TAGTCGTGAGGAAAACAGGCTTTATTTGAGTGTTTTAACTTTGGCAGAAATTGAAAAGGGAATAATAAAATGCCAGGAAC
CGGTAAGGCAGGAAAAGCTGCGAACCTGGCTTGAGTCTGATTTAATGGAGCGCTTCAAGCACAGAATATTACCTATAGAT
CTTGGGGTGGCAATAGCCTGGGGGCGTATTCAGGCTGAATCTGAAAAAAAGGGTACACCTATGCCCGTTATTGATGGGCT
TATAGCTGTCACAGGATTAGTGAATGACTGTACTGTAGTTACTCGAAATACAGGCGATATGCGCATGAGTCAGGTTCAGT
TATATAACCCTTGGGAAGACACGGCACCTTTCTGCGGTGTCAATTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6